VE-Cadherin Antibody (BV9)

Images

 
Immunocytochemistry/ Immunofluorescence: VE Cadherin Antibody (BV9) [NB600-1409] - Endothelial cells were fixed with 4% PAF (15 min, room temp), and then permeabilized with 0.5% TritionX-100 (3 min, room temperature). ...read more
Flow Cytometry: VE Cadherin Antibody (BV9) [NB600-1409] - HUVEC cells were stained with antibody BV9 in PBS/0.1% saponin for 1h at 4 degrees C.(Black- no stain, Red- isotype control, Blue-HM2032-BV9)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IA, ICC/IF, IHC, IHC-Fr, IP
Clone
BV9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

VE-Cadherin Antibody (BV9) Summary

Immunogen
The antibody recognizes the extracellular domain of the protein.
Localization
Intercellular junctions
Marker
Endothelial Cell Marker
Specificity
This monoclonal antibody (subclass IgG2a) binds to human VE-cadherin. Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CDH5
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10-1:100
  • Flow Cytometry 1:10-1:100
  • Functional 10-50 ug/ml
  • Immunoassay
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Anti-human VE-Cadherin antibody is useful for staining of frozen sections. The antibody can be used for Immunoassays, Western blots, Immunoprecipitation and for purification. Furthermore the antibody is useful for Flowcytometry and for inhibition in biological assays.
Positive Control
E-Cadherin Lysate (NBL1-09024)
Publications
Read Publications using
NB600-1409 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS with 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for VE-Cadherin Antibody (BV9)

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin

Background

Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VE-Cadherin Antibody (NB600-1409)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VE-Cadherin Antibody (NB600-1409) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for VE-Cadherin Antibody (NB600-1409)

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Bioinformatics

Gene Symbol CDH5
Entrez
Uniprot