VE-Cadherin Antibody (55-7H1) - BSA Free

Images

 
Separation of HUVEC cells stained using anti-CD144 (55-7H1) purified antibody (concentration in sample 5 ug/ml, GAM APC, red-filled) from HUVEC cells unstained by primary antibody (GAM APC, black-dashed) in Analysis ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
55-7H1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

VE-Cadherin Antibody (55-7H1) - BSA Free Summary

Additional Information
Clone 55-7H1 was used by HLDA to establish CD designation.
Immunogen
Human endothelial cells
Specificity
The mouse monoclonal antibody 55-7H1 recognizes a calcium-independent extracellular epitope on CD144 (VE-cadherin, cadherin 5), an adhesion molecule expressed on endothelial cells.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CDH5
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1-4 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for VE-Cadherin Antibody (55-7H1) - BSA Free

  • 7B4 antigen
  • 7B4
  • cadherin 5, type 2 (vascular endothelium)
  • cadherin 5, type 2, VE-cadherin (vascular epithelium)
  • Cadherin-5
  • CD144 antigen
  • CD144
  • CDH5
  • endothelial-specific cadherin
  • FLJ17376
  • Vascular endothelial cadherin
  • VECadherin
  • VE-Cadherin

Background

VE-cadherin (cadherin-5) is an endothelial specific adhesion molecule that is essential for the maintenance of endothelial barrier function and angiogenesis. VE-cadherin is linked to the actin cytoskeleton through a number of adaptor proteins including a-, â-, and ã-catenin. Cytoskeletal dynamics and phosphorylation regulate VE-cadherin mediated cell-cell adhesion. The cytosolic C-terminal Src kinase (Csk) binds via its SH2 domain to the phosphorylated tyrosine 658 site of VE-cadherin. This interaction regulates density dependent cell proliferation. Phosphorylation of tyrosine 658 has also been shown to lead to the uncoupling of p120-catenin from the cytoplasmic tail of VE-cadherin. This phosphorylation event is sufficient to maintain cells in a mesenchymal state during the cell invasion phase of angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDH5