Human VCAM-1/CD106 ELISA Kit (Colorimetric)

Human VCAM-1/CD106 ELISA Kit [NBP1-91177] - Standard curve for Human sVCAM-1 ELISA

Product Discontinued

Human VCAM-1/CD106 ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit has been transitioned to a new version, available here: DVC00
• Specificity: The assay detects both natural and recombinant human VCAM-1/CD106. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human VCAM-1/CD106 positive serum.
• Standard Curve Range: 3.15 - 100 ng/mL
• Sensitivity: 0.6 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.20%
• Intra-Assay: CV% < 3.10%
• Spike Recovery: The recovery ranged from 85% to 104% with an overall mean recovery of 89%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 100 uL (1:50 prediluted)g
• Kit Type: ELISA Kit (Colorimetric)
• Gene: VCAM1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human VCAM-1/CD106 ELISA Kit (Colorimetric)

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

The vascular cell adhesion molecule-1 (VCAM-1) or CD106 is a member of the immunoglobulin gene superfamily. The initial molecular cloning of VCAM-1 reported six extracellular Ig-like domains (6D VCAM-1). This 6D VCAM-1 arises due to alternative splicing from a seven-domain VCAM-1 (7D VCAM-1). 7D VCAM-1 is the dominant form expressed by cultured human endothelial cells. Domains 1 through 3 are highly homologous to domains 4 through 6, suggesting that they arose by gene duplication. VCAM-1 is not constitutively expressed on endothelium, but can be upregulated in vitro in response to LPS, TNF-a, and IL-1, as well as to IFN-gamma and IL-4. VCAM-1 is also present on tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts and myotubes. A soluble form of VCAM-1 (sVCAM-1) has been described. Soluble VCAM-1 levels have been found in the serum of healthy individuals and increased levels of sVCAM-1 can be detected in several diseases. Cancer: ovarian, gastric-intestinal, renal, bladder cancer, non-Hodgkin's lymphoma, breast cancer (cyst fluid). Autoimmune diseases: multiple sclerosis (cerebrospinal fluid), systemic sclerosis, systemic lupus erythematosus, rheumatoid arthritis. Infections: sepsis, meningitis, malaria. Inflammation: vasculitis, alcoholic cirrhosis, primary biliary cirrhosis, Wegener's granulomatosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for VCAM-1/CD106 ELISA Kit (NBP1-91177). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: VCAM1