Human VCAM-1/CD106 ELISA Kit (Colorimetric)

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Human VCAM-1/CD106 ELISA Kit [NBP1-91177] - Standard curve for Human sVCAM-1 ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
3.15 - 100 ng/mL
Sensitivity
0.6 ng/mL

Order Details

Human VCAM-1/CD106 ELISA Kit (Colorimetric) Summary

Specificity
The assay detects both natural and recombinant human VCAM-1/CD106. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human VCAM-1/CD106 positive serum.
Standard Curve Range
3.15 - 100 ng/mL
Sensitivity
0.6 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
5.20%
Intra-Assay
3.10%
Spike Recovery
The recovery ranged from 85% to 104% with an overall mean recovery of 89%. Please see protocol for more specific Spike Recovery details.
Sample Volume
100 uL (1:50 prediluted)
Kit Type
ELISA Kit (Colorimetric)
Gene
VCAM1

Applications/Dilutions

Application Notes
The human sVCAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sVCAM-1

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Alternate Names for Human VCAM-1/CD106 ELISA Kit (Colorimetric)

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

The vascular cell adhesion molecule-1 (VCAM-1) or CD106 is a member of the immunoglobulin gene superfamily. The initial molecular cloning of VCAM-1 reported six extracellular Ig-like domains (6D VCAM-1). This 6D VCAM-1 arises due to alternative splicing from a seven-domain VCAM-1 (7D VCAM-1). 7D VCAM-1 is the dominant form expressed by cultured human endothelial cells. Domains 1 through 3 are highly homologous to domains 4 through 6, suggesting that they arose by gene duplication. VCAM-1 is not constitutively expressed on endothelium, but can be upregulated in vitro in response to LPS, TNF-a, and IL-1, as well as to IFN-gamma and IL-4. VCAM-1 is also present on tissue macrophages, dendritic cells, bone marrow fibroblasts, myoblasts and myotubes. A soluble form of VCAM-1 (sVCAM-1) has been described. Soluble VCAM-1 levels have been found in the serum of healthy individuals and increased levels of sVCAM-1 can be detected in several diseases. Cancer: ovarian, gastric-intestinal, renal, bladder cancer, non-Hodgkin's lymphoma, breast cancer (cyst fluid). Autoimmune diseases: multiple sclerosis (cerebrospinal fluid), systemic sclerosis, systemic lupus erythematosus, rheumatoid arthritis. Infections: sepsis, meningitis, malaria. Inflammation: vasculitis, alcoholic cirrhosis, primary biliary cirrhosis, Wegener's granulomatosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol VCAM1
Entrez