VCAM-1/CD106 Antibody (EPR5047)

Product Discontinued

VCAM-1/CD106 Antibody (EPR5047) Summary

• Immunogen: A synthetic peptide corresponding to residues in mouse VCAM1 was used as an immunogen.
• Clonality: Monoclonal
• Host: Rabbit
• Gene: VCAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250-500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-100
  • Western Blot 1:1000-10000
• Application Notes: In Western blotting, detects band at ~100 kDa.

Reactivity Notes

Species cross-reactivity is based on Western Blot analysis.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS, 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for VCAM-1/CD106 Antibody (EPR5047)

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

Vascular cell adhesion protein 1 (VCAM1) is important in cell-cell recognition. It has been reported to function in leukocyte-endothelial cell adhesion, interact with the beta-1 integrin VLA4 on leukocytes, and mediate both adhesion and signal transduction. There are two isoforms; Isoform 1, a single-pass type I membrane protein, and isoform 2, a lipid-anchor (GPI-anchor). VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. It is also expressed in the bone marrow (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VCAM-1/CD106 Antibody (NBP1-95622)(3)

Publications using NBP1-95622

• Ankeny RF, Hinds MT, Nerem RM. Dynamic Shear Stress Regulation of Inflammatory and Thrombotic Pathways in Baboon Endothelial Outgrowth Cells. Tissue Eng Part A 2013-03-19 [PMID: 23406430] (WB, Baboon)
• Ruschkies L Analysis of microtubule-severing enzymes in the nervous system of Mus musculus (Linnaeus, 1754) Thesis 2019-01-01
• Mackesy Daniel Z, Goalstone Marc L. Extracellular signal-regulated kinase-5: Novel mediator of insulin and tumor necrosis factor alpha-stimulated vascular cell adhesion molecule-1 expression in vascular cells. J Diabetes. 2014-02-26 [PMID: 24460840]

FAQs for VCAM-1/CD106 Antibody (NBP1-95622). (Showing 1 - 1 of 1 FAQs).

1. Would you be able to send me more details about immunogen peptide for item NBP1-95622? I would just need to know if it was a n-term or c-term peptide?
   • With regards to the immunogen region for NBP1-95622, the C-terminus sequence of VCAM1 of mouse origin (accession # P29533) has been used for generating this antibody.

Bioinformatics

• Gene Symbol: VCAM1
• Entrez:
  • 7412(Human)
  • 22329(Mouse)
  • 25361(Rat)
• Uniprot:
  • P29533()