VAV2 Antibody

Images

 
Western Blot: VAV2 Antibody [NB100-895] - Staining of 293 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: VAV2 Antibody [NB100-895] - Staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VAV2 Antibody Summary

Immunogen
Peptide with sequence ETEAKYYRTLEDIEC corresponding to internal region according to NP_001127870.1, NP_003362.2.
Specificity
This antobody is expected to recognise both reported isoforms (NP_001127870.1 and NP_003362.2).
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VAV2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 1.5 ug/ml
Application Notes
WB: Approx. 100 kDa band observed in 293 lysate (predicted MW of 100 kDa band according to NP_003362). IHC: Paraffin embedded Human Colon.
Control
VAV2 Overexpression Lysate
Publications
Read Publication using NB100-895.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAV2 Antibody

  • guanine nucleotide exchange factor VAV2
  • oncogene VAV2
  • Protein vav-2
  • vav 2 guanine nucleotide exchange factor
  • vav 2 oncogene
  • VAV-2

Background

VAV2 is a ubiquitously expressed structural homolog of the VAV protooncogene that is expressed preferentially in hematopoetic cells. Both proteins are comprised of a Dbl homology (DH) domain with guanosine nucleotide exchange (GEF) activity exclusively directed towards Rho/Rac GTPases, a pleckstrin homology (PH) domain, a calponin-homology (CH) region, an acidic domain (AD) a zinc finger butterfly motif, two SH3 regions and one SH2 domain. GEF activity of RhoA family G proteins is induced by tyrosine phosphorylation in wild type VAV2, and is constitutively activated in N terminus deleted oncogene forms. Constitutive expression of a VAV2 oncoprotein may result in morphological alterations including highly enlarged cells in which karyokinesis and cytokinesis frequently are uncoupled.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAV2 Antibody (NB100-895)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VAV2
Entrez
Uniprot