VAV3 Antibody

Images

 
Western Blot: VAV3 Antibody [NB300-817] - Analysis of VAV3 in human bone marrow lysate (35 ug protein in RIPA buffer) using this antibody at 0.3 ug/ml.
Immunohistochemistry-Paraffin: VAV3 Antibody [NB300-817] - (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Data obtained from previous batch (goat).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VAV3 Antibody Summary

Immunogen
Peptide with sequence CSGEQGTLKLPEK corresponding to internal region according to NP_006104.4, NP_001073343.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VAV3
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.3-1 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 90 kDa band observed in human bone marrow lysates (calculated MW of 97.8 kDa band according to NP_006104).
Positive Control
VAV3 Lysate (NBP2-65176)
Publications
Read Publication using NB300-817.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAV3 Antibody

  • FLJ40431
  • guanine nucleotide exchange factor VAV3
  • vav 3 guanine nucleotide exchange factor
  • vav 3 oncogene
  • VAV-3

Background

VAV3, a third representative of the Vav family, participates in signal transduction processes. It is also involved in the activation of several members of the Rho family. This activity, when deregulated, leads to marked cytoskeletal changes and to alterations in the process of cell division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAV3 Antibody (NB300-817)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VAV3
Entrez
Uniprot