VAV3 Antibody

Immunocytochemistry/Immunofluorescence: VAV3 Antibody [NB300-817] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: VAV3 Antibody [NB300-817] - Staining of paraffin embedded Human Spleen. Antibody at 7.5 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Flow Cytometry: VAV3 Antibody [NB300-817] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: VAV3 Antibody [NB300-817] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

VAV3 Antibody Summary

• Immunogen: Peptide with sequence CSGEQGTLKLPEK corresponding to internal region according to NP_006104.4, NP_001073343.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_006104.4; NP_001073343.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: VAV3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10ug/ml
  • Immunohistochemistry 7.5ug/ml
  • Immunohistochemistry-Paraffin 7.5 ug/mL
  • Peptide ELISA Detection limit 1:32000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VAV3 Antibody

• FLJ40431
• guanine nucleotide exchange factor VAV3
• vav 3 guanine nucleotide exchange factor
• vav 3 oncogene
• VAV-3

Background

VAV3, a third representative of the Vav family, participates in signal transduction processes. It is also involved in the activation of several members of the Rho family. This activity, when deregulated, leads to marked cytoskeletal changes and to alterations in the process of cell division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAV3 Antibody (NB300-817)(1)

Publication using NB300-817

• Movilla N, Bustelo XR. Biological and regulatory properties of Vav-3, a new member of the Vav family of oncoproteins. Mol Cell Biol 1999-11-01 [PMID: 10523675]

Bioinformatics

• Gene Symbol: VAV3
• Entrez:
  • 10451(Human)
• Uniprot:
  • NP_006104.(Human)