VASA Antibody (2F9H5)

Images

 
Western Blot: VASA Antibody (2F9H5) [NBP1-51586] - Analysis using DDX4 mouse mAb against DDX4-hIgGFc transfected HEK293 cell lysate.
Immunocytochemistry/ Immunofluorescence: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
Immunohistochemistry-Paraffin: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization using DDX4 mouse mAb with DAB staining.
Flow Cytometry: VASA Antibody (2F9H5) [NBP1-51586] - Analysis of MSCS cells using DDX4 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2F9H5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

VASA Antibody (2F9H5) Summary

Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli.
Marker
Primordial Germ Cell Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DDX4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for VASA Antibody (2F9H5)

  • DDX4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
  • DEAD box protein 4
  • EC 3.6.1
  • EC 3.6.4.13
  • MGC111074
  • probable ATP-dependent RNA helicase DDX4
  • Vasa homolog
  • VASA
  • VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2379
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91012
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-65214
Species: Hu, Mu, Rt, Ma
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-2437
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB600-749
Species: Hu, Mu, Rt, Rb, Ze
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF6548
Species: Hu, Mu
Applications: IHC
NBP2-37398
Species: Hu
Applications: WB, ELISA, Flow, IHC, CyTOF-ready
NB300-232
Species: Mu, Rt, Po, Bv, Ch, Fe, Pa
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
H00388112-B01P
Species: Hu
Applications: WB, ELISA
AF1997
Species: Hu
Applications: WB, ChIP, ICC
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
AF4090
Species: Hu
Applications: WB, IHC, IP, Neut
NBP1-51586
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for VASA Antibody (NBP1-51586) (0)

There are no publications for VASA Antibody (NBP1-51586).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for VASA Antibody (NBP1-51586) (0)

There are no reviews for VASA Antibody (NBP1-51586). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for VASA Antibody (NBP1-51586). (Showing 1 - 1 of 1 FAQ).

  1. Has the staining of NTERA-2 cells using DDX4 mouse mAb been shown in a publication?
    • Despite being one of our best sellers, this antibody has not yet been cited in publications to our knowledge. However, we stand by the quality of our products and all our products are 100% guaranteed. We are always there to help in case you face any trouble in generating your desired outcome out of the use of our products.

Secondary Antibodies

 

Isotype Controls

Additional VASA Products

Bioinformatics Tool for VASA Antibody (NBP1-51586)

Discover related pathways, diseases and genes to VASA Antibody (NBP1-51586). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for VASA Antibody (NBP1-51586)

Discover more about diseases related to VASA Antibody (NBP1-51586).
 

Pathways for VASA Antibody (NBP1-51586)

View related products by pathway.

PTMs for VASA Antibody (NBP1-51586)

Learn more about PTMs related to VASA Antibody (NBP1-51586).

Blogs on VASA

There are no specific blogs for VASA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our VASA Antibody (2F9H5) and receive a gift card or discount.

Bioinformatics

Gene Symbol DDX4
Entrez
Uniprot