VAP-1/AOC3 Antibody [Unconjugated]

Western blot shows lysates of human lung tissue, human kidney tissue, human liver tissue, and human heart tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VAP-1/AOC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for VAP-1/AOC3 at approximately 95-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human lung tissue and heart tissue, loaded at 0.2 mg/mL. A specific band was detected for VAP‑1/AOC3 at approximately 123 kDa (as indicated) using 10 µg/mL of Goat Anti-Human VAP‑1/AOC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3957) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

VAP-1/AOC3 Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VAP‑1/AOC3
  Gly27-Asn763
  Accession # Q16853
• Specificity: Detects human VAP‑1/AOC3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse VAP-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AOC3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for VAP-1/AOC3 Antibody [Unconjugated]

• amine oxidase, copper containing 3 (vascular adhesion protein 1)
• AOC3
• Copper amine oxidase
• EC 1.4.3
• HPAO
• HPAOSSAO
• Semicarbazide-sensitive amine oxidase
• SSAO
• VAP1
• VAP-1
• VAP1EC 1.4.3.21
• VAP-1membrane primary amine oxidase
• Vascular adhesion protein 1

Background

Vascular Adhesion Protein-1 (VAP-1) is a copper amine oxidase with a topaquinone cofactor. VAP-1 is a Type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases (1, 2). VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H₂O₂ (3). The enzyme is sensitive to inhibition by semicarbazide. VAP-1 expression is highest in the endothelium of lung, heart, and intestine, but low in tissues such as brain, spleen, kidney, and liver (4). VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored (5). The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues (6). VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. The role of VAP-1 amine oxidase activity in this process is not fully defined, but it appears to be carbohydrate-dependent (7). VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases (8).

1. Kurkijärvi, R. et al. (1998) J. Immunol. 161:1549.
2. Gearing, A.J.H. and W. Newman (1993) Immunol. Today 14:506.
3. Lizcano, J.M. et al. (1998) Biochem. J. 331:69.
4. Smith, D.J. et al. (1998) J. Exp. Med. 188:17.
5. Jaakkala K. et al. (2000) Am. J. Pathol. 157:463.
6. Salmi, M. and J. Jalkanen (2001) Trends Immunol. 22:211.
7. Salmi, M. and J. Jalkanen (1996) J. Exp. Med. 183:569.
8. Dunkel, P. et al. (2008) Curr. Med. Chem. 15:1827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AOC3
• Uniprot:
  • Q16853()