CHT1 Antibody (62-2E8)

Images

 
Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
Clone
62-2E8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHT1 Antibody (62-2E8) Summary

Immunogen
Recombinant fusion protein from the C-terminal portion of the human CHT1 protein. [UniProt# Q9GZV3]
Localization
Membrane; Multi-pass membrane protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC5A7
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:500
Application Notes
This CHT1 antibody is useful for Immunohistochemistry frozen sections and paraffin embedded sections and Western blot. In Western blot a band can be seen at ~65 kDa representing CHT and a band can also be seen at ~45 kDa after deglycosylation with PNGase F.
Control
CHT1 Overexpression Lysate
Publications
Read Publications using
NB110-74570 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human, mouse and rat.Primate reactivity reported in scientific literature (PMID: 12820166)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50% Glycerol
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CHT1 Antibody (62-2E8)

  • CHT
  • CHT1MGC126299
  • hCHT
  • Hemicholinium-3-sensitive choline transporter
  • high affinity choline transporter 1
  • high affinity choline transporter; hemicholinium-3-sensitive cholinetransporter
  • MGC126300
  • solute carrier family 5 (choline transporter), member 7
  • Solute carrier family 5 member 7

Background

CHT1 (high affinity choline transporter 1) is a member of sodium:solute symporter (SSF) (TC 2.A.21) family that facilitates sodium/chloride-ion dependent import of choline from extracellular space to the neurons with high affinity. CHT1 is expressed in putamen, spinal cord and medulla, and is specific for cholinergic neurons. Acetyl choline is synthesized from choline and acetyl CoA by choline acetyltransferase in the cytoplasm of cholinergic neurons, and then incorporated into synaptic vesicles by VAChT (vesicular acetylcholine transporter). In the pre-synaptic terminals of cholinergic neurons, CHT1 mediates choline uptake which is the rate limiting step for acetyl-choline biosynthesis and is essential for post-natal viability. CHT1 been shown to be internalized in a clathrin dependent manner, whereas, the synaptic vesicles exocytosis enhances its cell surface expression; a mechanism that facilitates CHT1 functions at cellular surfaces with its predominant localization in the synaptic vesicles in cholinergic neurons. Moreover, Nedd4-2-mediated ubiquitination regulates CHT1 cell surface expression reflecting a possibility that treatments or drugs which inhibit CHT1-Nedd4-2 interactions might be useful for diseases involving decrease in acetylcholine level such as Alzheimer's disease. Moreover, polymorphic variation in CHT1 has been shown to predict early, subclinical measures of carotid atherosclerosis which may aid in understanding cholinergic-vagal processes potentially underlying atherosclerotic risk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-59378
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NBP1-30052
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl, IHC-WhMt
NBP1-60054
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Rb
Applications: WB
NBP1-58841
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ze
Applications: WB
NBP1-31329
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-51684
Species: Hu, Mu
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC
NBP1-89417
Species: Hu, Rt
Applications: WB, IHC, IHC-P, Single Cell Western
NBP1-26612
Species: Hu
Applications: IP (-), WB
NBP2-11567
Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-69368
Species: Hu, Po, Bv
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
H00005555-P01
Species: Hu
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NBP1-58930
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ze
Applications: WB
NB110-74570
Species: Hu, Mu, Rt, Pm
Applications: WB, IHC, IHC-Fr, IHC-P

Publications for CHT1 Antibody (NB110-74570)(3)

Reviews for CHT1 Antibody (NB110-74570) (0)

There are no reviews for CHT1 Antibody (NB110-74570). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for CHT1 Antibody (NB110-74570): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CHT1 Antibody (NB110-74570). (Showing 1 - 1 of 1 FAQs).

  1. One of our customers is interested in Anti-SLC5A7 antibody [62-2E8] (NB110-74570). Could you please provide further details about the immunogen sequence used to produce this antibody?
    • Unfortunately we cannot provide any information about the eiptope sequence as this is considered proprietary.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional CHT1 Products

Array NB110-74570

Bioinformatics Tool for CHT1 Antibody (NB110-74570)

Discover related pathways, diseases and genes to CHT1 Antibody (NB110-74570). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CHT1 Antibody (NB110-74570)

Discover more about diseases related to CHT1 Antibody (NB110-74570).
 

Pathways for CHT1 Antibody (NB110-74570)

View related products by pathway.

PTMs for CHT1 Antibody (NB110-74570)

Learn more about PTMs related to CHT1 Antibody (NB110-74570).
 

Research Areas for CHT1 Antibody (NB110-74570)

Find related products by research area.

Blogs on CHT1

There are no specific blogs for CHT1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our CHT1 Antibody (62-2E8) and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC5A7
Entrez
Uniprot