CHT1 Antibody (62-2E8)


Western Blot: CHT1 Antibody (62-2E8) [NB110-74570] - Western Blot Image of anti-CHT1 (62-2E8). Total protein from mouse brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% more
Immunohistochemistry: CHT1 Antibody (62-2E8) [NB110-74570] - Staining of neurons in rat basal forebrain

Product Details

Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC, IHC-Fr, IHC-P
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

CHT1 Antibody (62-2E8) Summary

Recombinant fusion protein from the C-terminal portion of the human CHT1 protein. [UniProt# Q9GZV3]
Membrane; Multi-pass membrane protein.
Tissue culture supernatant
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
50% Glycerol
0.05% Sodium Azide
Tissue culture supernatant


  • Western Blot 1:500
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Frozen 1:500
  • Immunohistochemistry-Paraffin 1:500
Application Notes
This CHT1 antibody is useful for Immunohistochemistry frozen sections and paraffin embedded sections and Western blot. In Western blot a band can be seen at ~65 kDa representing CHT and a band can also be seen at ~45 kDa after deglycosylation with PNGase F.
Read Publications using
NB110-74570 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human, mouse and rat.Primate reactivity reported in scientific literature (PMID: 12820166)

Alternate Names for CHT1 Antibody (62-2E8)

  • CHT
  • CHT1MGC126299
  • hCHT
  • Hemicholinium-3-sensitive choline transporter
  • high affinity choline transporter 1
  • high affinity choline transporter; hemicholinium-3-sensitive cholinetransporter
  • MGC126300
  • solute carrier family 5 (choline transporter), member 7
  • Solute carrier family 5 member 7


CHT1 (high affinity choline transporter 1) is a member of sodium:solute symporter (SSF) (TC 2.A.21) family that facilitates sodium/chloride-ion dependent import of choline from extracellular space to the neurons with high affinity. CHT1 is expressed in putamen, spinal cord and medulla, and is specific for cholinergic neurons. Acetyl choline is synthesized from choline and acetyl CoA by choline acetyltransferase in the cytoplasm of cholinergic neurons, and then incorporated into synaptic vesicles by VAChT (vesicular acetylcholine transporter). In the pre-synaptic terminals of cholinergic neurons, CHT1 mediates choline uptake which is the rate limiting step for acetyl-choline biosynthesis and is essential for post-natal viability. CHT1 been shown to be internalized in a clathrin dependent manner, whereas, the synaptic vesicles exocytosis enhances its cell surface expression; a mechanism that facilitates CHT1 functions at cellular surfaces with its predominant localization in the synaptic vesicles in cholinergic neurons. Moreover, Nedd4-2-mediated ubiquitination regulates CHT1 cell surface expression reflecting a possibility that treatments or drugs which inhibit CHT1-Nedd4-2 interactions might be useful for diseases involving decrease in acetylcholine level such as Alzheimer's disease. Moreover, polymorphic variation in CHT1 has been shown to predict early, subclinical measures of carotid atherosclerosis which may aid in understanding cholinergic-vagal processes potentially underlying atherosclerotic risk.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHT1 Antibody (NB110-74570)(3)

Reviews for CHT1 Antibody (NB110-74570) (0)

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Product General Protocols

View specific protocols for CHT1 Antibody (NB110-74570): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CHT1 Antibody (NB110-74570). (Showing 1 - 1 of 1 FAQs).

  1. One of our customers is interested in Anti-SLC5A7 antibody [62-2E8] (NB110-74570). Could you please provide further details about the immunogen sequence used to produce this antibody?
    • Unfortunately we cannot provide any information about the eiptope sequence as this is considered proprietary.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol SLC5A7

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