USP4 Antibody - BSA Free

Immunoprecipitation: USP4 Antibody [NB100-2868] - Detection of Human USP4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-USP4 antibody NB100-2868 used for IP at 3 mcg/mg lysate. USP4 was also immunoprecipitated by rabbit anti-USP4 antibody NB100-2869, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

USP4 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human Ubiquitin Specific Protease 4 using the numbering given in entry AAB72237.1 (GeneID 7375).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Not Recommended for Western Blot. Use rabbit anti-USP4 Antibody NB 100-2868.
• Control:

  USP4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP4 Antibody - BSA Free

• Deubiquitinating enzyme 4
• EC 3.1.2.15
• EC 3.4.19.12
• MGC149848
• MGC149849
• ubiquitin carboxyl-terminal esterase 4
• ubiquitin carboxyl-terminal hydrolase 4
• ubiquitin specific peptidase 4 (proto-oncogene)
• ubiquitin specific protease 4 (proto-oncogene)
• ubiquitin thioesterase 4
• Ubiquitin thiolesterase 4
• ubiquitin-specific processing protease 4
• Ubiquitin-specific-processing protease 4
• Ubiquitous nuclear protein homolog
• UNP
• Unph
• UNPUnph
• USP4

Background

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP4 Antibody (NB100-2868)(1)

Publication using NB100-2868

• Yang Y, Xia F, Hermance N et al. A cytosolic ATM/NEMO/RIP1 complex recruits TAK1 to mediate the NF-kappaB and p38 mitogen-activated protein kinase (MAPK)/MAPK-activated protein 2 responses to DNA damage. Mol Cell Biol 2011-07-01 [PMID: 21606198]

Bioinformatics

• Gene Symbol: USP4
• Entrez:
  • 7375(Human)
• Uniprot:
  • AAB72237.1(Human)