TRIM21/SSA1 Antibody

Images

 
Western Blot: TRIM21/SSA1 Antibody [NBP1-33548] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548] - Paraffin-embedded DLD-1 xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: TRIM21/SSA1 Antibody [NBP1-33548] - Immunoprecipitation of SSA1 protein from 293T whole cell extracts using 5 ug of SSA1 antibody [C1C3]. Western blot analysis was performed using SSA1 antibody ...read more

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRIM21/SSA1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SSA1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (86%), Pig (81%), Bovine (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM21/SSA1 Antibody

  • 52 kDa ribonucleoprotein autoantigen Ro/SS-A
  • 52 kDa Ro/SSA
  • E3 ubiquitin-protein ligase TRIM21
  • EC 6.3.2.-
  • RNF81
  • RNF81RING finger protein 81
  • ro(SS-A)
  • Ro52
  • RO52SS-A
  • Sicca syndrome antigen A
  • Sjoegren syndrome type A antigen
  • Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
  • SSA1
  • SSA1SSA
  • TRIM21
  • tripartite motif containing 21,52 kDa Ro protein
  • tripartite motif-containing 21
  • Tripartite motif-containing protein 21

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-48002
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IHC-P, IP, PA
NBP1-86998
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57100
Species: Hu
Applications: ICC/IF
MAB2358
Species: Hu, Mu
Applications: IHC, ICC
NBP2-03433
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34234
Species: Hu
Applications: ICC/IF, IHC-Fr, IHC-P
NB200-540
Species: Mu
Applications: WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB500-526
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-31898
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89649
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87914
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-57487
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-33548
Species: Hu, RM
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for TRIM21/SSA1 Antibody (NBP1-33548) (0)

There are no publications for TRIM21/SSA1 Antibody (NBP1-33548).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TRIM21/SSA1 Antibody (NBP1-33548) (0)

There are no reviews for TRIM21/SSA1 Antibody (NBP1-33548). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TRIM21/SSA1 Antibody (NBP1-33548) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TRIM21/SSA1 Products

Bioinformatics Tool for TRIM21/SSA1 Antibody (NBP1-33548)

Discover related pathways, diseases and genes to TRIM21/SSA1 Antibody (NBP1-33548). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRIM21/SSA1 Antibody (NBP1-33548)

Discover more about diseases related to TRIM21/SSA1 Antibody (NBP1-33548).
 

Pathways for TRIM21/SSA1 Antibody (NBP1-33548)

View related products by pathway.

PTMs for TRIM21/SSA1 Antibody (NBP1-33548)

Learn more about PTMs related to TRIM21/SSA1 Antibody (NBP1-33548).
 

Research Areas for TRIM21/SSA1 Antibody (NBP1-33548)

Find related products by research area.

Blogs on TRIM21/SSA1

There are no specific blogs for TRIM21/SSA1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRIM21/SSA1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TRIM21
Entrez
Uniprot