TRIM21/SSA1 Antibody

Images

 
Western Blot: TRIM21/SSA1 Antibody [NBP1-33548] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SSA1 antibody [C1C3] diluted ...read more
Immunocytochemistry/ Immunofluorescence: TRIM21/SSA1 Antibody [NBP1-33548] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: TRIM21/SSA1 Antibody [NBP1-33548] - DLD-1 xenograft , using SSA1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: TRIM21/SSA1 Antibody [NBP1-33548] - Immunoprecipitation of SSA1 protein from 293T whole cell extracts using 5 ug of SSA1 antibody [C1C3]. Western blot analysis was performed using SSA1 antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRIM21/SSA1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SSA1. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus
Predicted Species
Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockdown Validated -Reported in scientific literature (PMID:32029896)
  • Western Blot 1:500-1:3000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-33548 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • IP
    1 publication
  • KD
    1 publication
  • WB
    1 publication

Reactivity Notes

Cat (86%), Pig (81%), Bovine (82%). Use in Mouse reported in scientific literature (PMID:32029896).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for TRIM21/SSA1 Antibody

  • 52 kDa ribonucleoprotein autoantigen Ro/SS-A
  • 52 kDa Ro/SSA
  • E3 ubiquitin-protein ligase TRIM21
  • EC 6.3.2.-
  • rhTRIM21
  • RNF81
  • RNF81RING finger protein 81
  • ro(SS-A)
  • Ro52
  • RO52SS-A
  • Sicca syndrome antigen A
  • Sjoegren syndrome type A antigen
  • Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
  • SSA1
  • SSA1SSA
  • TRIM21
  • tripartite motif containing 21,52 kDa Ro protein
  • tripartite motif-containing 21
  • Tripartite motif-containing protein 21

Background

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM21/SSA1 Antibody (NBP1-33548)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Co-IP, IHC, IP, KD, WB.


Filter By Application
Co-IP
(1)
IHC
(1)
IP
(1)
KD
(1)
WB
(1)
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Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-33548 Applications Species
Ho S, Chaput D, Sinkey R et al. Proteomic studies of human placentas reveal partnerships associated with preeclampsia, diabetes, gravidity, and labor medRxiv 2023-07-27 (IHC, Human) IHC Human
Liu RX, Gu RH, Li ZP et al. Trim21 depletion alleviates bone loss in osteoporosis via activation of YAP1/?-catenin signaling Bone research 2023-10-26 [PMID: 37884520] (Co-IP, WB, Mouse) Co-IP, WB Mouse
Li JT, Yin M, Wang D et al. BCAT2-mediated BCAA catabolism is critical for development of pancreatic ductal adenocarcinoma Nat. Cell Biol. 2020-02-01 [PMID: 32029896] (KD, IP, Mouse, Human) KD, IP Mouse, Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM21
Entrez
Uniprot