USP21 Antibody

Western Blot: USP21 Antibody [NBP2-75701] - Analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: USP21 Antibody [NBP2-75701] - Staining USP21 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: USP21 Antibody [NBP2-75701] - Analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. Counter stained with hematoxylin.

Flow Cytometry: USP21 Antibody [NBP2-75701] - Analysis of SH-SY-5Y cells with USP21 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: USP21 Antibody [NBP2-75701] - Staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: USP21 Antibody [NBP2-75701] - Analysis of paraffin-embedded human liver cancer tissue using anti-USP21 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: USP21 Antibody [NBP2-75701] - Analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. Counter stained with hematoxylin.

Product Discontinued

USP21 Antibody Summary

• Immunogen: Synthetic peptide within Human USP21 aa 1-50 / 565. (SwissProt: Q9UK80 Human; SwissProt: Q9QZL6 Mouse; SwissProt: B2GUX4 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP21 Antibody

• Deubiquitinating enzyme 21
• EC 3.1.2.15
• EC 3.4.19.12
• ESTM28
• MGC3394
• ubiquitin carboxyl-terminal hydrolase 21
• ubiquitin specific peptidase 21
• ubiquitin specific protease 21
• ubiquitin thioesterase 21
• Ubiquitin thiolesterase 21
• ubiquitin-specific processing protease 21
• ubiquitin-specific protease 16
• Ubiquitin-specific-processing protease 21
• USP16
• USP21
• USP23
• USP23NEDD8-specific protease
• W53272

Background

USP21 encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: USP21