USP21 Antibody

Product Discontinued

USP21 Antibody Summary

• Immunogen: Peptide with sequence GRTREPPVNIQP-C corresponding to N-Terminus according to NP_036607.3.
• Epitope: internal region
• Specificity: Reported variants represent identical protein: NP_001014443.1 NP_036607.3
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: USP21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments in human thyroid, thymus and tonsil lysates gave no specific signal but low background.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for USP21 Antibody

• Deubiquitinating enzyme 21
• EC 3.1.2.15
• EC 3.4.19.12
• ESTM28
• MGC3394
• ubiquitin carboxyl-terminal hydrolase 21
• ubiquitin specific peptidase 21
• ubiquitin specific protease 21
• ubiquitin thioesterase 21
• Ubiquitin thiolesterase 21
• ubiquitin-specific processing protease 21
• ubiquitin-specific protease 16
• Ubiquitin-specific-processing protease 21
• USP16
• USP21
• USP23
• USP23NEDD8-specific protease
• W53272

Background

This gene encodes a member of the C19 peptidase family, also known as family 2 of ubiquitin carboxy-terminal hydrolases. The encoded protein cleaves ubiquitin from ubiquitinated proteins for recycling in intracellular protein degradation. The encoded protein is also able to release NEDD8, a ubiquitin-like protein, from NEDD8-conjugated proteins. This gene has been referred to as USP16 and USP23 but is now known as USP21. Alternatively spliced transcript variants have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP21 Antibody (NBP1-20996)(1)

Publication using NBP1-20996

• Gong L, Kamitani T, Millas S, Yeh ET. Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins. J Biol Chem 2000-05-12 [PMID: 10799498]

Bioinformatics

• Gene Symbol: USP21
• Entrez:
  • 27005(Human)
  • 30941(Mouse)
  • 688466(Rat)
• Uniprot:
  • NP_036607.3(Human)