USP15 Antibody

Images

 
Genetic Strategies: Knockdown Validated: USP15 Antibody [NB110-40690] - USP15 knockdown with three siRNAs in LN-229 and (b) two siRNAs in LN-428. Image collected and cropped by CiteAb from the following ...read more
Western Blot: USP15 Antibody [NB110-40690] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti- USP15 antibody NB110-40690 ...read more
Immunohistochemistry-Paraffin: USP15 Antibody [NB110-40690] - Sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- USP15 used at a dilution of 1:200 ...read more
Biological Strategies: Western Blot: USP15 Antibody [NB110-40690] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

USP15 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 960 and the C-terminus of human Ubiquitin Specific Peptidase 15 (AAD41086.1).
Predicted Species
Bovine (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
Application Notes
Efficiency of IP is low. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB110-40690 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for USP15 Antibody

  • Deubiquitinating enzyme 15
  • EC 3.1.2.15
  • EC 3.4.19.12
  • Gcap18
  • KIAA0529
  • KIAA0529ubiquitin carboxyl-terminal hydrolase 15
  • MGC131982
  • MGC149838
  • MGC74854
  • ubiquitin specific peptidase 15
  • ubiquitin specific protease 15
  • ubiquitin thioesterase 15
  • Ubiquitin thiolesterase 15
  • Ubiquitin-specific-processing protease 15
  • Ubp109
  • UNPH-2
  • UNPH4
  • USP15

Background

USP15 belongs to a large group of deubiquitinating enzymes that are responsible for processing inactive Ub precursors by removing Ub from cellular adducts. USP15 is a CSN associated deubiquitinylase with a Zn-finger that is essential for the cleavage of poly-Ub chains, important in the rescue of E3 ligase Rbx1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-40690
Species: Hu, Mu, Rt, Bv, Xp
Applications: WB, IHC, IHC-P, IP, KD

Publications for USP15 Antibody (NB110-40690)(3)

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Bioinformatics

Gene Symbol USP15
Entrez
Uniprot