U937 human histiocytic lymphoma cell line was stained with Goat Anti-Human IL‑31 RA Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2769, filled histogram) or isotype control antibody (Catalog # AB-108-C, ...read more
IL‑31 RA was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human IL‑31 RA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2769) at 5 µg/mL for 3 ...read more
IL‑31 RA was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Goat Anti-Human IL‑31 RA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2769) at 15 µg/mL for 3 ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-31RA Antibody [Unconjugated]
class I cytokine receptor
Cytokine receptor-like 3
GP130 like receptor
Gp130-like monocyte receptor
IL-31 receptor subunit alpha
IL-31R subunit alpha
interleukin 31 receptor A
interleukin-31 receptor subunit alpha
soluble type I cytokine receptor CRL3
The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon-gamma and bacterial lipopolysaccharides (1‑3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3‑6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, canine and bovine IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356‑745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their co-expression with OSM-R determines a cell’s response to IL‑31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).
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Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
Bando, T. et al. (2006) Neuroscience 142:1263.
Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
Diveu, C. et al. (2004) Eur. Cytokine. Netw. 15:291.
Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.