USP11 Antibody

Western Blot: USP11 Antibody [NB100-1251] - Western blot analysis of USP11 in Jurkat lysate using NB100-1251 at 1 ug/ ml.

Western Blot: USP11 Antibody [NB100-1251] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

USP11 Antibody Summary

• Immunogen: Peptide with sequence CSSPPSSEFMDVN corresponding to C-Terminus according to NP_004642.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: USP11
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 120 kDa band observed in human testis lysates and Jurkat lysate is reported in scientific literature (PMID: 12084015).
• Control:

  Jurkat Whole Cell Lysate
  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for USP11 Antibody

• Deubiquitinating enzyme 11
• EC 3.1.2.15
• EC 3.4.19.12
• ubiquitin carboxyl-terminal hydrolase 11
• ubiquitin specific peptidase 11
• ubiquitin specific protease 11
• Ubiquitin Thioesterase 11
• Ubiquitin thiolesterase 11
• ubiquitin-specific processing protease 11
• Ubiquitin-specific-processing protease 11
• UHX1
• UHX1ubiquitin carboxyl-terminal hydrolase, X-linked
• USP11

Background

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for USP11 Antibody (NB100-1251)(1)

Publication using NB100-1251

• Ideguchi H, Ueda A, Tanaka M et al. Structural and functional characterization of the USP11 deubiquitinating enzyme, which interacts with the RanGTP-associated protein RanBPM. Biochem J 2002-10-01 [PMID: 12084015]

Bioinformatics

• Gene Symbol: USP11
• Entrez:
  • 8237(Human)
• Uniprot:
  • NP_004642.(Human)