UNC13D/Munc 13-4 Antibody (1222B) [Unconjugated]

Western blot shows lysates of NK-92 human natural killer lymphoma cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB8966) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for UNC13D at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

UNC13D was detected in immersion fixed NK-92 human natural killer lymphoma cell line using Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB8966) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UNC13D at approximately 112 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human UNC13D Monoclonal Antibody (Catalog # MAB8966). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clone: 1222B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

UNC13D/Munc 13-4 Antibody (1222B) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: KLH-coupled N-terminal human UNC13D peptide of an alternative isoform described in PMID:24842371
  Accession # EAW89322
• Specificity: Detects the alternate form of human UNC13D in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UNC13D
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 2-25 ug/mL
  • Simple Western 0.5 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for UNC13D/Munc 13-4 Antibody (1222B) [Unconjugated]

• FHL3
• HLH3
• HPLH3
• Munc13-4
• Munc13-4protein unc-13 homolog D
• unc-13 homolog D (C. elegans)
• UNC13D

Background

UNC13D (also Munc13-4) is a 123 kDa cytoplasmic and peripheral membrane protein that is expressed at highest levels in hematopoietic tissues.  UNC13D appears to play a role in vesicle maturation during exocytosis and its expression is obligatory for exocytosis of cytolytic granules from NK and T cells. A point mutation in intron 1 of UNC13D causes familial hematophagocytic lymphohistiocytosis type3, a rare autosomal recessive immune deficiency. The mutation disrupts transcription factor binding and prevents expression of an alternative isoform that is required for lymphocyte cytotoxicity. The conventional and alternative isoforms are identical from amino acid 40-1090 but have distinct N-terminal segments.  It is unknown whether mice express the alternative isoform. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UNC13D/Munc 13-4 Antibody (MAB8966)(2)

Publications using MAB8966

• Xiaojuan Zhang, Quanbin Xu, Zhike Zi, Zeyu Liu, Chun Wan, Lauren Crisman et al. Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications Developmental Cell 2020-12-08 [PMID: 33296682]
• Donatella Galgano, Tayebeh Soheili, Matthias Voss, Lamberto Torralba-Raga, Bianca Tesi, Frank Cichocki et al. Alternative UNC13D Promoter Encodes a Functional Munc13-4 Isoform Predominantly Expressed in Lymphocytes and Platelets Frontiers in Immunology 2020-06-09 [PMID: 32582217] (Western Blot, Human)

FAQs for UNC13D/Munc 13-4 Antibody (MAB8966). (Showing 1 - 1 of 1 FAQs).

1. I'm looking Munc13-4 N terminal antibody with related epitope sequence.
   • Unfortunately we do not epitope map our antibodies, but we do have a whole list of antibodies against your target depending on species reactivity and application you want to use them for. Please see this link to our MUNC 13-4 antibodies. All of the immunogen information that is not proprietary, or known should be presented on the datasheet. Often times a range will be provided where the peptide falls within, or if it was raised against a larger recombinant protein as is the case for the one you inquired about we do not know exactly where the binding occurs.

Bioinformatics

• Gene Symbol: UNC13D
• Uniprot:
  • EAW89322()