ULBP-1 Antibody (237104) [Unconjugated]

A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 /MULT-1 Monoclonal Antibody (Catalog # MAB2588, filled histogram) or isotype control antibody (MAB006, open histogram) followed by anti-Rat IgG PE-conjugated secondary antibody (F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 237104
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

ULBP-1 Antibody (237104) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
• Specificity: Detects mouse ULBP‑1 /MULT-1.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: ULBP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-1 Antibody (237104) [Unconjugated]

• ALCAN-beta
• MULT-1
• N2DL1
• N2DL-1
• NKG2D ligand 1
• NKG2DL
• NKG2DL1
• RAET1I
• RAET1Ialcan-beta
• Retinoic acid early transcript 1I
• UL16 binding protein 1
• UL16-binding protein 1
• ULBP1
• ULBP-1

Background

ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8⁺ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (K_D = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ULBP-1 Antibody (MAB2588)(16)

Publications using MAB2588

• Zhang, H;Tang, Q;Miao, Y;Wang, J;Yuan, Z;Huang, X;Zhu, Y;Nong, C;Li, G;Cui, R;Huang, X;Zhang, L;Yu, Q;Jiang, Z; Group 1 innate lymphoid cell activation via recognition of NKG2D and liver resident macrophage MULT-1: Collaborated roles in triptolide induced hepatic immunotoxicity in mice Ecotoxicology and environmental safety 2024-02-10 [PMID: 38342011] (Flow Cytometry, Immunocytochemistry, Mouse)
• G D'Alessand, F Antonangel, F Marrocco, A Porzia, C Lauro, A Santoni, C Limatola Gut microbiota alterations affect glioma growth and innate immune cells involved in tumor immunosurveillance in mice Eur. J. Immunol., 2020-03-01;0(0):. 2020-03-01 [PMID: 32034922] (Flow Cytometry, Mouse)
• Y Nakamura, S Miyagawa, S Yoshida, S Sasawatari, T Toyofuku, K Toda, Y Sawa Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model Sci Rep, 2019-07-25;9(1):10840. 2019-07-25 [PMID: 31346220] (Flow Cytometry, Mouse)
• M Alvarez, F Simonetta, J Baker, A Pierini, AS Wenokur, AR Morrison, WJ Murphy, RS Negrin Regulation of murine NK cell exhaustion through the activation of the DNA damage repair pathway JCI Insight, 2019-06-18;5(0):. 2019-06-18 [PMID: 31211693] (Functional Assay, Mouse)
• L Ma, M Han, Z Keyoumu, H Wang, S Keyoumu Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma Med. Sci. Monit., 2017-04-25;23(0):1980-1991. 2017-04-25 [PMID: 28439064] (Flow Cytometry, Mouse)
• Li Hua, Mingli Fang, Boqi Dong, Sheng Guo, Cuiyun Cui, Jiwei Liu et al. Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice Oncotarget 12/13/2016 [PMID: 27448968]
• Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine. PLoS ONE, 2015-12-02;10(12):e0143224. 2015-12-02 [PMID: 26630176] (Flow Cytometry, Mouse)
• Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015-01-29;135(5):1311-9. 2015-01-29 [PMID: 25634359] (Flow Cytometry, Western Blot, Mouse)
• Tietje A, Li J, Yu X, Wei Y MULT1E/mIL-12: a novel bifunctional protein for natural killer cell activation. Gene Ther, 2014-02-27;21(5):468-75. 2014-02-27 [PMID: 24572784] (ELISA Development, Mouse)
• Paolino , Magdalen, Choidas , Axel, Wallner , Stephani, Pranjic , Blanka, Uribesalgo , Iris, Loeser , Stefanie, Jamieson , Amanda M, Langdon , Wallace, Ikeda , Fumiyo, Fededa , Juan Pab, Cronin , Shane J, Nitsch , Roberto, Schultz-Fademrecht , Carsten, Eickhoff , Jan, Menninger , Sascha, Unger , Anke, Torka , Robert, Gruber , Thomas, Hinterleitner , Reinhard, Baier , Gottfrie, Wolf , Dominik, Ullrich , Axel, Klebl , Bert M, Penninger , Josef M The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells. Nature, 2014-02-19;507(7493):508-12. 2014-02-19 [PMID: 24553136] (Flow Cytometry, Mouse)
• Jung , Heiyoun, Hsiung , Benjamin, Pestal , Kathleen, Procyk , Emily, Raulet , David H RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry. J Exp Med, 2012-11-19;209(13):2409-22. 2012-11-19 [PMID: 23166357] (Flow Cytometry, Mouse)
• Yoshida S, Mohamed RH, Kajikawa M, Koizumi J, Tanaka M, Fugo K, Otsuka N, Maenaka K, Yagita H, Chiba H, Kasahara M Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair. J. Immunol., 2012-03-07;188(8):3972-9. 2012-03-07 [PMID: 22403443] (IHC-Fr, Mouse)
• Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood, 2009-02-06;113(24):6120-7. 2009-02-06 [PMID: 19202127] (Flow Cytometry, Mouse)
• Timothy J. Nice, Laurent Coscoy, David H. Raulet Posttranslational regulation of the NKG2D ligand Mult1 in response to cell stress Journal of Experimental Medicine 2009-02-16 [PMID: 19171762] (Flow Cytometry, Mouse)
• Kotturi HS, Li J, Branham-O'Connor M, Stickel SL, Yu X, Wagner TE, Wei Y Tumor cells expressing a fusion protein of MULT1 and Fas are rejected in vivo by apoptosis and NK cell activation. Gene Ther., 2008-05-08;15(19):1302-10. 2008-05-08 [PMID: 18463688] (Flow Cytometry, Mouse)
• Vilarinho S, Ogasawara K, Nishimura S, Lanier LL, Baron JL Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus. Proc. Natl. Acad. Sci. U.S.A., 2007-11-08;104(46):18187-92. 2007-11-08 [PMID: 17991774] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: ULBP1