Recombinant Human NKp30 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human NKp30 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When recombinant human B7-H6 Fc Chimera is immobilized at 1 μg/mL (100 µL/well), the concentration of recombinant human NKp30 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 8-40 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human NKp30/NCR3 protein
Human NKp30
(Leu19 - Thr138)
Accession # Q05D23
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Leu19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NCR3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
40 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
54-60 kDa, reducing conditions
Publications
Read Publications using
1849-NK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKp30 Fc Chimera Protein, CF

  • Activating natural killer receptor p30
  • CD337 antigen
  • CD337
  • CD337activating NK-A1 receptor
  • LY117,1C7NK-p30
  • lymphocyte antigen 117
  • natural cytotoxicity triggering receptor 3
  • Natural killer cell p30-related protein
  • NCR3
  • NKp30
  • NKp30MALS

Background

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK-mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM‑bearing accessory protein, CD3 zeta, occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK-mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.

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1849-NK
Species: Hu
Applications: Bioactivity

Publications for NKp30/NCR3 (1849-NK)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Bacteria.

We have publications tested in 4 applications: Bioassay, Enzyme Assay, Flow Cytometry, Neutralization.


Filter By Application
Bioassay
(2)
Enzyme Assay
(1)
Flow Cytometry
(13)
Neutralization
(1)
All Applications
Filter By Species
Human
(15)
Mouse
(1)
Bacteria
(1)
All Species
Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 1849-NK Applications Species
A Tremblay-M, S Coenraads, Z Kiani, FP Dupuy, NF Bernard Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function BMC Immunol., 2019;20(1):8. 2019 [PMID: 30696399] (Flow Cytometry, Human) Flow Cytometry Human
Impaired NK-mediated regulation of T-cell activity in multiple sclerosis is reconstituted by IL-2 receptor modulation Proc Natl Acad Sci USA, 2016;113(21):E2973-82. 2016 [PMID: 27162345] (Enzyme Assay, Human) Enzyme Assay Human
Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Onfelt B Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells. J Immunol, 2015;195(7):3374-81. 2015 [PMID: 26320254] (Flow Cytometry, Human) Flow Cytometry Human
Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure. PLoS ONE, 2015;10(8):e0134568. 2015 [PMID: 26241657] (Neutralization, Human) Neutralization Human
Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1. PLoS ONE, 2015;10(5):e0125544. 2015 [PMID: 25950680] (Flow Cytometry, Human) Flow Cytometry Human
Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014;192(11):5332-42. 2014 [PMID: 24790151] (Bioassay, Human) Bioassay Human
Siewiera J, El Costa H, Tabiasco J, Berrebi A, Cartron G, Le Bouteiller P, Jabrane-Ferrat N Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013;9(4):e1003257. 2013 [PMID: 23592985] (Bioassay, Human) Bioassay Human
Reiners K, Kessler J, Sauer M, Rothe A, Hansen H, Reusch U, Hucke C, Kohl U, Durkop H, Engert A, von Strandmann E Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients. Mol Ther, 2013;21(4):895-903. 2013 [PMID: 23459515] (Flow Cytometry, Human) Flow Cytometry Human
Alvarez-Breckenridge, Christop, Yu, Jianhua, Price, Richard, Wojton, Jeffrey, Pradarelli, Jason, Mao, Hsiaoyin, Wei, Min, Wang, Yan, He, Shun, Hardcastle, Jayson, Fernandez, Soledad, Kaur, Balveen, Lawler, Sean E, Vivier, Eric, Mandelboim, Ofer, Moretta, Alessand, Caligiuri, Michael, Chiocca, E Antoni NK cells impede glioblastoma virotherapy through NKp30 and NKp46 natural cytotoxicity receptors. Nat Med, 2012;18(12):1827-34. 2012 [PMID: 23178246] (Flow Cytometry, Human) Flow Cytometry Human
Bellucci R, Nguyen HN, Martin A, Heinrichs S, Schinzel AC, Hahn WC, Ritz J Tyrosine kinase pathways modulate tumor susceptibility to natural killer cells. J. Clin. Invest., 2012;122(7):2369-83. 2012 [PMID: 22684105] (Flow Cytometry, Human) Flow Cytometry Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol NCR3
Uniprot