ULBP-1 Antibody (237104) [Alexa Fluor® 647]

Images

 
A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse ULBP-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB2588R, filled histogram) or isotype control antibody (Catalog # IC006R, open ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
237104
Clonality
Monoclonal
Host
Rat
Conjugate
Alexa Fluor 647
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

ULBP-1 Antibody (237104) [Alexa Fluor® 647] Summary

Immunogen
BaF3 mouse pro-B cell line transfected with mouse ULBP‑1 /MULT-1
Specificity
Detects mouse ULBP‑1 /MULT-1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ULBP1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-1 Antibody (237104) [Alexa Fluor® 647]

  • ALCAN-beta
  • MULT-1
  • N2DL1
  • N2DL-1
  • NKG2D ligand 1
  • NKG2DL
  • NKG2DL1
  • RAET1I
  • RAET1Ialcan-beta
  • Retinoic acid early transcript 1I
  • UL16 binding protein 1
  • UL16-binding protein 1
  • ULBP1
  • ULBP-1

Background

ULBP-1, also known as MULT-1 (mouse UL16-binding protein-like Transcript 1), is a 53 kDa, MHC Class I-like molecule that belongs to the mouse family of NKG2D ligands (1‑4). It is a type I transmembrane glycoprotein that is synthesized as a 334 amino acid (aa) precursor. It contains a 25 aa signal sequence, a 186 aa extracellular region, a 19 aa transmembrane segment and a 104 aa cytoplasmic tail (2). The extracellular region contains an alpha 1 and alpha 2 like domain with two intrachain disulfide bonds. ULBP-1 is distantly related to other human and mouse NKG2D ligands, and more distantly related to the MHC class I proteins (3). Unlike most NKG2D ligands, transcripts for ULBP-1 have been detected in a wide variety of mouse tissues and tumor cells lines (3). The receptor for ULBP-1 is NKG2D, a 35 kDa C-type lectin that is found on mouse NK cells, activated CD8+ T cells, epidermal gamma δ T cells, and activated macrophages (1, 5, 6, 7). Recombinant ULBP-1 protein binds to NKG2D with high affinity (KD = 6 nM) (2). Although an activating receptor, general cellular responses to NKG2D ligation depend upon the isoform of NKG2D and the cell type (5). Exposure to immobilized ULBP-1 or ULBP-1-transfected cells elicits IFN-gamma production by NK cells (3). Ectopic expression of ULBP-1 on the RMA mouse tumor cell line leads to tumor rejection in syngeneic mice (3).

  1. Raulet, D.H. (2003) Nat. Rev. Immunol. 3:781.
  2. Carayannopoulos, L. et al. (2002) J. Immunol. 169:4079.
  3. Diefenbach, A. et al. (2003) Eur. J. Immunol. 33:381.
  4. Krmpotic, A. et al. (2005) J. Exp. Med. 201:211.
  5. Diefenbach, A. et al. (2002) Nat. Immunol. 3:1142.
  6. Ho, E.L. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6320.
  7. Carayannopoulos, L.N. et al. (2002) Eur. J. Immunol. 32:597.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ULBP1