Recombinant Human ULBP-5 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ULBP-5 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human ULBP-5 (Catalog # 7149-UL) binds to Recombinant Human (rh) NKG2D/CD314 Fc Chimera (Catalog # 1299-NK) with an ED 50 of 1.50-15.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human ULBP-5 protein Met1-Gly210, with a C-terminal 6-His tag
• Accession #: NP_001001788
• N-terminal Sequence: Asp29
• Protein/Peptide Type: Recombinant Proteins
• Gene: RAET1G
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 24-28 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ULBP-5 Protein, CF

• RAET1G
• retinoic acid early transcript 1G protein
• retinoic acid early transcript 1G pseudogene
• retinoic acid early transcript 1G
• ULBP5
• ULBP-5

Background

ULBP-5 (cytomegalovirus glycoprotein UL16 binding protein 5), also called RAET1G (retinoic acid early transcript 1G), is a 37 kDa member of a family of cell-surface proteins that function as ligands for human NKG2D (1-3). When engaged, NKG2D activates cytolytic activity and/or cytokine production by effector cells that express it, such as NK cells, NKT cells, gamma δ T cells, and CD8 + alpha beta T cells (3-5). Human ULBP-5 mRNA encodes a 25 amino acid (aa) signal sequence, a 198 aa extracellular domain (ECD), a 20 aa transmembrane domain, and a 91 aa cytoplasmic sequence. Transmembrane, predicted soluble (RAET1G2), and glycosylphosphatidylinositol (GPI)-anchored isoforms are identical through aa 210, while a shorter soluble form (RAET1G3) lacks aa 118-154 (3-5). Secreted, cell surface and intracellular ULBP-5 have been described (3-8). While most family members are GPI-anchored, only ULBP-5 and ULBP-4/RAET1E express a transmembrane form (4, 5). Within the ECD, ULBP-5 possesses two MHC Class I alpha domains and shares ~93% aa sequence identity with ULBP-2/RAET1H plus ULBP-6/RAET1L, and 30-60% aa identity with remaining family members (1, 5). Rodent NKG2D ligands Rae-1 alpha -epsilon are, like ULBP proteins, distantly related to MHC class I proteins, but ULBP and Rae-1 family proteins do not share significant sequence identity (2). ULBP-5 mRNA expression is low or undetected in many tissues, but increased expression is reported in epithelial tissues and human decidua (5-8). ULBP-5 is expressed on some breast, epithelial and hematopoietic tumor cells and has been implicated in tumor surveillance (4-6).

1. Radosavljevic, M. et al. (2002) Genomics 79:114.
2. Kondo, M. et al. (2010) Immunogenetics 62:441.
3. Ohashi, M. et al. (2010) J. Biol. Chem. 285:16408.
4. Cao, W. et al. (2008) Int. Immunol. 20:981.
5. Bacon, L. et al. (2004) J. Immunol. 173:1078.
6. Eagle, R.A. et al. (2009) PLoS ONE 4:e4503.
7. Wittenbrink, M. et al. (2009) Eur. J. Immunol. 39:1642.
8. Apps, R. et al. (2008) Hum. Reprod. 23:2535.

Bioinformatics

• Gene Symbol: RAET1G
• Uniprot:
  • NP_001001788()