ULBP-4/RAET1E Antibody (709116) [Unconjugated]

Images

 
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ULBP‑4/RAET1E Monoclonal Antibody (Catalog # MAB6285) followed by HRP-conjugated ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human ULBP‑4/RAET1E Monoclonal Antibody (Catalog # MAB6285, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more
ULBP‑4/RAET1E was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human ULBP‑4/RAET1E Monoclonal Antibody (Catalog # MAB6285) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
709116
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

ULBP-4/RAET1E Antibody (709116) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ULBP‑4/RAET1E
Gly30-Asp225
Accession # Q8TD07
Specificity
Detects human ULBP‑4/RAET1E in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP‑1 or rhULBP-3 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
RAET1E
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • CyTOF-ready
Publications
Read Publications using
MAB6285 in the following applications:

  • 3 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ULBP-4/RAET1E Antibody (709116) [Unconjugated]

  • bA350J20.7
  • LETAL
  • LETALRAE-1-like transcript 4
  • Lymphocyte effector toxicity activation ligand
  • MGC125309
  • N2DL4
  • N2DL-4
  • NKG2D ligand 4
  • NKG2DL4
  • RAET1E
  • RAET1E2
  • retinoic acid early transcript 1ERL-4
  • RL-4
  • ULBP4
  • ULBP-4
  • ULBP4MGC125308

Background

ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), also called RAET1E (retinoic acid early transcript 1E), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the ULBP/RAET1 family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only ULBP-4/RAET1E and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human ULBP-4 mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, ULBP-4 shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha -epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of ULBP‑4 mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). ULBP-4 is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). ULBP-4 binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma δ T cells, direct ULBP-4 binding to both TCR gamma δ and NKG2D has been demonstrated (6). ULBP-4 is also thought to function as a minor histocompatibility antigen in humans (1).
  1. Radosavljevic, M. et al. (2002) Genomics 79:114.
  2. Kondo, M. et al. (2010) Immunogenetics 62:441.
  3. Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
  4. Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
  5. Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
  6. Kong, Y. et al. (2009) Blood 114:310.
  7. Bacon, L. et al. (2004) J. Immunol. 173:1078.
  8. Cao, W. et al. (2008) Int. Immunol. 20:981.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ULBP-4/RAET1E Antibody (MAB6285)(3)

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Bioinformatics

Gene Symbol RAET1E
Uniprot