MICB Antibody (236511) [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant human MICB
Accession # CAI18747
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Protein A or G purified from hybridoma culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
- ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
- ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
- ELISA Standard (Matched Pair)
- Flow Cytometry 0.25 ug/10^6 cells
- Knockout Validated 0.25 ug/10^6 cells
- Western Blot 1 ug/mL
ELISA Capture: Human MICB Antibody (Catalog # MAB1599)
ELISA Detection: Human MICB Biotinylated Antibody (Catalog # BAF1599)
Standard: Recombinant Human MICB Fc Chimera (Catalog # 1599-MB)
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for MICB Antibody (236511) [Unconjugated]
- MHC class I chain-related protein B
- MHC class I mic-B antigen
- MHC class I polypeptide-related sequence B
- PERB11.2MHC class I-like molecule PERB11.2-IMX
- stress inducible class I homolog
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).
- Groh, V. et al. (2001) Nature Immunol. 2:255.
- Stephens, H. (2001) Trends Immunol. 22:378.
- Bauer, S. et al. (1999) Science 285:727.
- Groh, V. et al. (2002) Nature 419:734.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- Salih, H. et al. (2003) Blood 102:1389.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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