MICB Antibody (236511) [Unconjugated]

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

MICB knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the MICB knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ELISA(Cap), KO
• Clone: 236511
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MICB Antibody (236511) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MICB
  Ala23-Gly298
  Accession # CAI18747
• Specificity: Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human MICB Antibody (Catalog # MAB1599)
  ELISA Detection: Human MICB Biotinylated Antibody (Catalog # BAF1599)
  Standard: Recombinant Human MICB Fc Chimera (Catalog # 1599-MB)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICB Antibody (236511) [Unconjugated]

• MHC class I chain-related protein B
• MHC class I mic-B antigen
• MHC class I polypeptide-related sequence B
• MICB
• MIC-B
• PERB11.2MHC class I-like molecule PERB11.2-IMX
• stress inducible class I homolog

Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. Salih, H. et al. (2003) Blood 102:1389.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICB Antibody (MAB1599)(36)

Publications using MAB1599

• J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022;13(1):1606. 2022 [PMID: 35338135] (Western Blot, Human)
• S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021;12(9):836. 2021 [PMID: 34482362] (ICC, Human)
• Y Zhu, J Xie, J Shi Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis Bmc Biology, 2021;19(1):140. 2021 [PMID: 34325694] (Western Blot, Human)
• E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021;17(5):e1008807. 2021 [PMID: 33939764] (Flow Cytometry, Human)
• E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021;12(1):2061. 2021 [PMID: 33824318] (Flow Cytometry, Human)
• M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020;12(7):. 2020 [PMID: 32698530] (Flow Cytometry, Human)
• SH Chang, HJ Kim, CG Park Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens Cells, 2020;9(5):. 2020 [PMID: 32443511] (Flow Cytometry, Human)
• M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production Cell. Mol. Immunol., 2019;0(0):. 2019 [PMID: 31471588] (Flow Cytometry, Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019;5(0):. 2019 [PMID: 31184599] (IHC, Human)
• YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Flow Cytometry, Human)
• MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha Purine nucleotide metabolism regulates expression of the human immune ligand MICA J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29279329] (Flow Cytometry, Human)
• Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017;7(1):8661. 2017 [PMID: 28819195] (Flow Cytometry, Human)
• C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells Cell Death Dis, 2017;8(8):e2973. 2017 [PMID: 28771222] (Flow Cytometry, Human)
• AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017;8(19):31959-31976. 2017 [PMID: 28404876] (Flow Cytometry, Human)
• O Berhani, D Nachmani, R Yamin, D Schmiedel, Y Bar-On, O Mandelboim Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5' Untranslated Region J. Immunol., 2017;0(0):. 2017 [PMID: 28356383] (Flow Cytometry, Human)
• Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells Sci Rep, 2016;6(0):32470. 2016 [PMID: 27671170] (Flow Cytometry, Human)
• Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol, 2015;195(2):736-48. 2015 [PMID: 26071561] (Neutralization, Human)
• Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer, 2015;15(0):351. 2015 [PMID: 25933805] (Flow Cytometry, Human)
• Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015;135(5):1311-9. 2015 [PMID: 25634359] (FACS, Human)
• Esteso G, Luzon E, Sarmiento E, Gomez-Caro R, Steinle A, Murphy G, Carbone J, Vales-Gomez M, Reyburn H Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA. J Immunol, 2014;193(3):1344-52. 2014 [PMID: 24973455] (ELISA Development, Human)
• Nachmani , Daphna, Gutschner , Tony, Reches , Adi, Diederichs , Sven, Mandelboim , Ofer RNA-binding proteins regulate the expression of the immune activating ligand MICB. Nat Commun, 2014;5(0):4186. 2014 [PMID: 24924487] (Flow Cytometry, Human)
• Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prod'homme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation. PLoS Pathog, 2014;10(5):e1004058. 2014 [PMID: 24787765] (Neutralization, Human)
• Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathog, 2014;10(2):e1003963. 2014 [PMID: 24586166] (Flow Cytometry, Human)
• Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells. Cancer Res, 2012;72(21):5463-72. 2012 [PMID: 22915757] (Flow Cytometry, Human)
• Nielsen N, Odum N, Urso B, Lanier LL, Spee P Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A. PLoS ONE, 2012;7(2):e31959. 2012 [PMID: 22384114] (Flow Cytometry, Human)
• Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction. Blood, 2011;117(18):4778-86. 2011 [PMID: 21406724] (Flow Cytometry, Human)
• Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells. PLoS ONE, 2011;6(2):e16899. 2011 [PMID: 21364924] (Western Blot, Human)
• Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus. J. Exp. Med., 2011;208(1):13-21. 2011 [PMID: 21173105] (Flow Cytometry, Human)
• Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection. Blood, 2010;115(25):5170-9. 2010 [PMID: 20393128] (Flow Cytometry, Human)
• Ohashi M, Eagle RA, Trowsdale J Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform. J. Biol. Chem., 2010;285(22):16408-15. 2010 [PMID: 20304922] (Flow Cytometry, Human)
• Wu JD, Atteridge CL, Wang X, Seya T, Plymate SR Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation. Clin. Cancer Res., 2009;15(2):632-40. 2009 [PMID: 19147769] (Flow Cytometry, Mouse)
• Cerboni C, Ardolino M, Santoni A, Zingoni A Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 2009;113(13):2955-64. 2009 [PMID: 19124832] (Neutralization, Human)
• Boutet P, Aguera-Gonzalez S, Atkinson S, Pennington CJ, Edwards DR, Murphy G, Reyburn HT, Vales-Gomez M Cutting edge: the metalloproteinase ADAM17/TNF-alpha-converting enzyme regulates proteolytic shedding of the MHC class I-related chain B protein. J. Immunol., 2009;182(1):49-53. 2009 [PMID: 19109134] (ELISA Development, Human)
• Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol. Immunol., 2008;45(15):3917-25. 2008 [PMID: 18657862] (Flow Cytometry, Human)
• Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine. Biochem. Biophys. Res. Commun., 2008;370(4):578-83. 2008 [PMID: 18395517] (Flow Cytometry, Human)
• Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B. Cancer Res., 2005;65(23):11136-45. 2005 [PMID: 16322264] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MICB
• Uniprot:
  • CAI18747()