MICB Antibody (236511) [Unconjugated]

Images

 
MICB knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) ...read more
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap), KO
Clone
236511
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

MICB Antibody (236511) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICB
Ala23-Gly298
Accession # CAI18747
Specificity
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MICB
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Knockout Validated 0.25 ug/10^6 cells
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human MICB Antibody (Catalog # MAB1599)
ELISA Detection: Human MICB Biotinylated Antibody (Catalog # BAF1599)
Standard: Recombinant Human MICB Fc Chimera (Catalog # 1599-MB)
Publications
Read Publications using
MAB1599 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICB Antibody (236511) [Unconjugated]

  • MHC class I chain-related protein B
  • MHC class I mic-B antigen
  • MHC class I polypeptide-related sequence B
  • MICB
  • MIC-B
  • PERB11.2MHC class I-like molecule PERB11.2-IMX
  • stress inducible class I homolog

Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Salih, H. et al. (2003) Blood 102:1389.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MICB Antibody (MAB1599)(27)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA Development, FACS, Flow Cytometry, Neutralization, Western Blot.


Filter By Application
ELISA Development
(2)
FACS
(1)
Flow Cytometry
(20)
Neutralization
(3)
Western Blot
(1)
All Applications
Filter By Species
Human
(26)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using MAB1599 Applications Species
YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Flow Cytometry, Human) Flow Cytometry Human
MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha Purine nucleotide metabolism regulates expression of the human immune ligand MICA J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29279329] (Flow Cytometry, Human) Flow Cytometry Human
Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20 Sci Rep, 2017;7(1):8661. 2017 [PMID: 28819195] (Flow Cytometry, Human) Flow Cytometry Human
C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells Cell Death Dis, 2017;8(8):e2973. 2017 [PMID: 28771222] (Flow Cytometry, Human) Flow Cytometry Human
AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition Oncotarget, 2017;8(19):31959-31976. 2017 [PMID: 28404876] (Flow Cytometry, Human) Flow Cytometry Human
O Berhani, D Nachmani, R Yamin, D Schmiedel, Y Bar-On, O Mandelboim Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5' Untranslated Region J. Immunol., 2017;0(0):. 2017 [PMID: 28356383] (Flow Cytometry, Human) Flow Cytometry Human
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells Sci Rep, 2016;6(0):32470. 2016 [PMID: 27671170] (Flow Cytometry, Human) Flow Cytometry Human
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol, 2015;195(2):736-48. 2015 [PMID: 26071561] (Neutralization, Human) Neutralization Human
Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids. BMC Cancer, 2015;15(0):351. 2015 [PMID: 25933805] (Flow Cytometry, Human) Flow Cytometry Human
Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity. J Invest Dermatol, 2015;135(5):1311-9. 2015 [PMID: 25634359] (FACS, Human) FACS Human
Show All 27 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1599U
Alexa Fluor 405 FAB1599V
Alexa Fluor 488 FAB1599G
Alexa Fluor 488 FAB1599G
Alexa Fluor 594 FAB1599T
Alexa Fluor 647 FAB1599R
Alexa Fluor 700 FAB1599N
Alexa Fluor 700 FAB1599N
Alexa Fluor 750 FAB1599S
Allophycocyanin FAB1599A
Allophycocyanin FAB1599A
Phycoerythrin FAB1599P
Phycoerythrin FAB1599P

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Bioinformatics

Gene Symbol MICB
Uniprot