MICB Antibody (236511) [Unconjugated]


K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more
MICB knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) ...read more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap), KO

Order Details

MICB Antibody (236511) [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human MICB
Accession # CAI18747
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human MICB Antibody (Catalog # MAB1599)
ELISA Detection: Human MICB Biotinylated Antibody (Catalog # BAF1599)
Standard: Recombinant Human MICB Fc Chimera (Catalog # 1599-MB)
Read Publications using
MAB1599 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICB Antibody (236511) [Unconjugated]

  • MHC class I chain-related protein B
  • MHC class I mic-B antigen
  • MHC class I polypeptide-related sequence B
  • MICB
  • MIC-B
  • PERB11.2MHC class I-like molecule PERB11.2-IMX
  • stress inducible class I homolog


MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Salih, H. et al. (2003) Blood 102:1389.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MICB Antibody (MAB1599)(36)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ELISA Development, FACS, Flow Cytometry, ICC, IHC, Neutralization, Western Blot.

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Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using MAB1599 Applications Species
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022;13(1):1606. 2022 [PMID: 35338135] (Western Blot, Human) Western Blot Human
S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021;12(9):836. 2021 [PMID: 34482362] (ICC, Human) ICC Human
Y Zhu, J Xie, J Shi Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis Bmc Biology, 2021;19(1):140. 2021 [PMID: 34325694] (Western Blot, Human) Western Blot Human
E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021;17(5):e1008807. 2021 [PMID: 33939764] (Flow Cytometry, Human) Flow Cytometry Human
E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021;12(1):2061. 2021 [PMID: 33824318] (Flow Cytometry, Human) Flow Cytometry Human
M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020;12(7):. 2020 [PMID: 32698530] (Flow Cytometry, Human) Flow Cytometry Human
SH Chang, HJ Kim, CG Park Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens Cells, 2020;9(5):. 2020 [PMID: 32443511] (Flow Cytometry, Human) Flow Cytometry Human
M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production Cell. Mol. Immunol., 2019;0(0):. 2019 [PMID: 31471588] (Flow Cytometry, Human) Flow Cytometry Human
DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019;5(0):. 2019 [PMID: 31184599] (IHC, Human) IHC Human
YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Flow Cytometry, Human) Flow Cytometry Human
Show All 36 Publications.

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Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Gene Symbol MICB