New pricing — Effective July 1, 2022 -

Amidst rising costs across all areas of our business, and aggressive attempts to implement cost savings without sacrificing quality, we announce the need to implement a cost increase starting July 1, 2022. If you have questions, please contact your sales representative.

MICB Antibody (236511) [Unconjugated]

Images

 
MICB knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) ...read more
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ELISA(Cap), KO
Clone
236511
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

MICB Antibody (236511) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICB
Ala23-Gly298
Accession # CAI18747
Specificity
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MICB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human MICB Antibody (Catalog # MAB1599)
ELISA Detection: Human MICB Biotinylated Antibody (Catalog # BAF1599)
Standard: Recombinant Human MICB Fc Chimera (Catalog # 1599-MB)
Publications
Read Publications using
MAB1599 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICB Antibody (236511) [Unconjugated]

  • MHC class I chain-related protein B
  • MHC class I mic-B antigen
  • MHC class I polypeptide-related sequence B
  • MICB
  • MIC-B
  • PERB11.2MHC class I-like molecule PERB11.2-IMX
  • stress inducible class I homolog

Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Salih, H. et al. (2003) Blood 102:1389.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB139
Species: Hu
Applications: CostimT, CyTOF-ready, Flow, Neut, WB
NBP2-61871
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NB120-6405
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
DY1298
Species: Hu
Applications: ELISA
MAB1380
Species: Hu
Applications: Block, CyTOF-ready, Flow
H00003126-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
NB100-64775
Species: Pm, Bv, Hu, Mu, Pm
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P (-), IP
NBP2-45316
Species: Hu, Pm
Applications: Flow, ICC/IF, IF, IHC, IHC-P, WB
NBP2-50419
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IP, WB
MAB1517
Species: Hu
Applications: Block, CyTOF-ready, Flow
247-ILB/CF
Species: Hu
Applications: BA
NBP2-14093
Species: Hu
Applications: IHC, IHC-P, WB
NBP3-07168
Species: Pm, Ca, Pm, Hu, Pm, Sq
Applications: Flow, ICC/IF
NB100-104
Species: Ca, Hu, Ma, Mu, Rt
Applications: ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, WB
MAB97861
Species: Hu
Applications: Flow, IHC, IHC-P
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB

Publications for MICB Antibody (MAB1599)(35)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Development, FACS, Flow Cytometry, ICC, Neutralization, Western Blot.


Filter By Application
ELISA Development
(2)
FACS
(1)
Flow Cytometry
(25)
ICC
(1)
Neutralization
(3)
Western Blot
(3)
All Applications
Filter By Species
Human
(34)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using MAB1599 Applications Species
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022;13(1):1606. 2022 [PMID: 35338135] (Western Blot, Human) Western Blot Human
S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition Cell Death & Disease, 2021;12(9):836. 2021 [PMID: 34482362] (ICC, Human) ICC Human
Y Zhu, J Xie, J Shi Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis Bmc Biology, 2021;19(1):140. 2021 [PMID: 34325694] (Western Blot, Human) Western Blot Human
E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing PloS Pathogens, 2021;17(5):e1008807. 2021 [PMID: 33939764] (Flow Cytometry, Human) Flow Cytometry Human
E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain Nature Communications, 2021;12(1):2061. 2021 [PMID: 33824318] (Flow Cytometry, Human) Flow Cytometry Human
M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D Viruses, 2020;12(7):. 2020 [PMID: 32698530] (Flow Cytometry, Human) Flow Cytometry Human
SH Chang, HJ Kim, CG Park Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens Cells, 2020;9(5):. 2020 [PMID: 32443511] (Flow Cytometry, Human) Flow Cytometry Human
M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production Cell. Mol. Immunol., 2019;0(0):. 2019 [PMID: 31471588] (Flow Cytometry, Human) Flow Cytometry Human
YS Lee, W Heo, J Nam, YH Jeung, J Bae The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells J. Radiat. Res., 2018;0(0):. 2018 [PMID: 29518205] (Flow Cytometry, Human) Flow Cytometry Human
MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha Purine nucleotide metabolism regulates expression of the human immune ligand MICA J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29279329] (Flow Cytometry, Human) Flow Cytometry Human
Show All 35 Publications.

Reviews for MICB Antibody (MAB1599) (0)

There are no reviews for MICB Antibody (MAB1599). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MICB Antibody (MAB1599) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MICB Products

Bioinformatics Tool for MICB Antibody (MAB1599)

Discover related pathways, diseases and genes to MICB Antibody (MAB1599). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MICB Antibody (MAB1599)

Discover more about diseases related to MICB Antibody (MAB1599).
 

Pathways for MICB Antibody (MAB1599)

View related products by pathway.

PTMs for MICB Antibody (MAB1599)

Learn more about PTMs related to MICB Antibody (MAB1599).

Blogs on MICB.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MICB Antibody (236511) [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol MICB
Uniprot