Glutathione Synthetase Antibody (JB95-33)

Western Blot: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.Positive control:Lane 1: SiHa Lane 2: Mouse colonLane 3: Rat liver

Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.

Flow Cytometry: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody (JB95-33) [NBP2-75513] - Analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC
• Clone: JB95-33
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Glutathione Synthetase Antibody (JB95-33) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human Glutathione Synthetase aa 301-420 / 474. (SwissProt: P48637 Human; SwissProt: P51855 Mouse; SwissProt: P46413 Rat)
• Localization: Cytosol. Extracellular region or secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GSS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Glutathione Synthetase Antibody (JB95-33)

• EC 6.3.2.3
• Glutathione synthase
• glutathione synthetase
• GSH synthetase
• GSHS
• GSH-S
• MGC14098

Background

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage byfree radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as ahomodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion ofgamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Synthetase Antibody (NBP2-75513)(1)

Publication using NBP2-75513

• Deka B, Sarma P, Manna P et al. Alliin Enriched Standardized Fraction of Allium Hookeri Regulates the Glucose Homeostasis Via Upregulating Hepatic Glutathione Pool SSRN Electronic Journal 2023-02-17 (WB, Rat)

Bioinformatics

• Gene Symbol: GSS