UDP glucose dehydrogenase Antibody - BSA Free

Western Blot: UDP glucose dehydrogenase Antibody [NBP2-20798] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UGDH antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: UDP glucose dehydrogenase Antibody [NBP2-20798] - UGDH antibody detects UGDH protein at cytosol on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. UGDH antibody dilution: 1:500.

Western Blot: UDP glucose dehydrogenase Antibody [NBP2-20798] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 7. 5% SDS PAGE gel, diluted at 1:10000.

Western Blot: UDP glucose dehydrogenase Antibody [NBP2-20798] - UGDH antibody detects UGDH protein by Western blot analysis. A. 50 ug Rat liver lysate/extract7. 5 % SDS-PAGEUGDH antibody dilution: 1:2000.

Immunocytochemistry/ Immunofluorescence: UDP glucose dehydrogenase Antibody [NBP2-20798] - UDP glucose dehydrogenase antibody detects UDP glucose dehydrogenase protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: UDP glucose dehydrogenase stained by UDP glucose dehydrogenase antibody (NBP2-20798) diluted at 1:500.

Western Blot: UDP glucose dehydrogenase Antibody [NBP2-20798] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UGDH antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

UDP glucose dehydrogenase Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human UDP glucose dehydrogenase. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UGDH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for UDP glucose dehydrogenase Antibody - BSA Free

• EC 1.1.1.22
• GDH
• UDPGDHUDP-glucose dehydrogenase
• UDP-Glc dehydrogenase
• UDP-GlcDH
• UDP-glucose 6-dehydrogenase
• UGD
• uridine diphospho-glucose dehydrogenase

Background

The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: UGDH
• Uniprot:
  • O60701(Human)