UCP2 Antibody (3F1B9)

Images

 
Western Blot: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis using UCP2 mAb against human UCP2 (AA: 1-309) recombinant protein. (Expected MW is 36.1 kDa)
Immunocytochemistry/ Immunofluorescence: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis of Hela cells using UCP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis of Hela cells using UCP2 mouse mAb (green) and negative control (red).
Western Blot: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis using UCP2 mAb against HEK293 (1) and UCP2 hIgGFc transfected HEK293 (2) cell lysate.
ELISA: UCP2 Antibody (3F1B9) [NBP2-52582] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
Clone
3F1B9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

UCP2 Antibody (3F1B9) Summary

Immunogen
Purified recombinant fragment of human UCP2 (AA: 1-309) expressed in E. Coli.
Isotype
IgG2 Alpha
Clonality
Monoclonal
Host
Mouse
Gene
UCP2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry
  • CyTOF-ready
Theoretical MW
33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
UCP2 Lysate (NBP2-64811)
UCP2 Lysate (NBP2-65514)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for UCP2 Antibody (3F1B9)

  • mitochondrial uncoupling protein 2
  • SLC25A8
  • SLC25A8BMIQ4
  • Solute carrier family 25 member 8
  • UCP 2
  • UCP2
  • UCPH
  • uncoupling protein 2 (mitochondrial, proton carrier)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCP2 Antibody (NBP2-52582). (Showing 1 - 1 of 1 FAQ).

  1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
    • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol UCP2
Entrez