Rat UCP1 ELISA Kit (Colorimetric)

ELISA: Rat UCP1 ELISA Kit (Colorimetric) [NBP2-82465] - Samples were spiked with high concentrations of Rat UCP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat UCP1 ELISA Kit (Colorimetric) [NBP2-82465] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL

Rat UCP1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat UCP1 in samples. No Significant cross-reactivity or interference between Rat UCP1 and analogues was observed.
• Standard Curve Range: 0.16 - 10 ng/mL
• Sensitivity: 0.09 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.48
• Intra-Assay: CV% < 4.75
• Spike Recovery: 91-107%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: UCP1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat UCP1 ELISA Kit (Colorimetric)

• SLC25A7
• SLC25A7mitochondrial brown fat uncoupling protein 1
• Solute carrier family 25 member 7
• Thermogenin
• UCP 1
• UCP
• UCP1
• uncoupling protein 1 (mitochondrial, proton carrier)

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for UCP1 ELISA Kit (NBP2-82465). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: UCP1