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UCH-L1/PGP9.5 Antibody (UCHL1/775) [DyLight 350]

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Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clone
UCHL1/775
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47932AF350
Alexa Fluor 405
NBP2-47932AF405
Alexa Fluor 488
NBP2-47932AF488
Alexa Fluor 532
NBP2-47932AF532
Alexa Fluor 594
NBP2-47932AF594
Alexa Fluor 647
NBP2-47932AF647
Alexa Fluor 700
NBP2-47932AF700
Alexa Fluor 750
NBP2-47932AF750
Biotin
NBP2-47932B
DyLight 405
NBP2-47932V
DyLight 488
NBP2-47932G
DyLight 550
NBP2-47932R
DyLight 594
NBP2-47932DL594
DyLight 650
NBP2-47932C
DyLight 680
NBP2-47932FR
DyLight 755
NBP2-47932IR
FITC
NBP2-47932F
HRP
NBP2-47932H
Janelia Fluor 549
NBP2-47932JF549
Janelia Fluor 646
NBP2-47932JF646
Unconjugated
NBP2-44795-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-44795-0.1mg
IHC-Prediluted
NBP2-48315
Azide and BSA Free
NBP2-47932-0.1mg
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UCH-L1/PGP9.5 Antibody (UCHL1/775) [DyLight 350] Summary

Immunogen
Recombinant human UCHL1 protein
Localization
Cytoplasmic. Endoplasmic Reticulum membrane
Marker
pan-Neuronal Marker
Specificity
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
UCHL1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human, Rat. Shows a broad species reactivity.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for UCH-L1/PGP9.5 Antibody (UCHL1/775) [DyLight 350]

  • EC 3.4.19.12
  • EC 6.-
  • Neuron cytoplasmic protein 9.5
  • PARK5
  • PGP 9.5
  • PGP9.5
  • PGP9.5Uch-L1
  • PGP95
  • ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
  • ubiquitin carboxyl-terminal hydrolase isozyme L1
  • ubiquitin C-terminal hydrolase
  • Ubiquitin thioesterase L1
  • UCHL1
  • UCH-L1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: alpha-Synuclein Antibody [NBP2-15365] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody diluted at 1:2000 and competitor's antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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NB300-270
Simple Western: Park7/DJ-1 Antibody [NB300-270] - Simple Western lane view shows a specific band for Park7 (DJ-1) in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: Park7/DJ-1 Antibody [NB300-270] - Lysates of the human RPE cell lines ARPE-19 (lane 1) and D407 (lane 2), the mouse RPE cell line B6-RPE07 (lane 3), mouse primary RPE (lane 4) and mouse brain lysates (lane 5) were harvested and analyzed by immunoblot assay with DJ-1 antibody. The DJ-1 signal varied in intensity in each RPE cell culture. Each lane contained 20 ug of protein. Protein loads were confirmed in replicate blots probed with GAPDH. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB300-270] - Representative confocal micrographs of ARPE-19 monolayers plated on Transwells(R) and labeled with antibodies to DJ-1 (G,J) and COX IV (H,K). Under baseline conditions, there is very little colocalization between DJ-1 and COX IV, as observed in overlaid images (I); DJ-1 is mostly distributed through the cytoplasm (arrows). Upon oxidative stress induced by incubation with 400 uM H2O2 for 1 hr, DJ-1 staining is increased (arrowheads) in ARPE-19 (J) cultures. In cultures treated with H2O2 some DJ-1 re-distributed to mitochondria (arrowheads) and displayed significant colocalization with COX IV in overlaid images (L). Scale bar = 10 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0067983), licensed under a CC-BY licence.
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UCH-L1/PGP9.5
Antibody (UCHL1/775) ...
NBP2-47932UV
UCH-L1/PGP9.5 Antibody (UCHL1/775) [DyLight 350]
Species: Hu, Rt
Applications: WB, IHC, IHC-P

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47932AF350
Alexa Fluor 405 NBP2-47932AF405
Alexa Fluor 488 NBP2-47932AF488
Alexa Fluor 532 NBP2-47932AF532
Alexa Fluor 594 NBP2-47932AF594
Alexa Fluor 647 NBP2-47932AF647
Alexa Fluor 700 NBP2-47932AF700
Alexa Fluor 750 NBP2-47932AF750
Biotin NBP2-47932B
DyLight 350 NBP2-47932UV
DyLight 405 NBP2-47932V
DyLight 488 NBP2-47932G
DyLight 550 NBP2-47932R
DyLight 594 NBP2-47932DL594
DyLight 650 NBP2-47932C
DyLight 680 NBP2-47932FR
DyLight 755 NBP2-47932IR
FITC NBP2-47932F
HRP NBP2-47932H
Janelia Fluor 549 NBP2-47932JF549
Janelia Fluor 646 NBP2-47932JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol UCHL1