Ubiquitin B Antibody (UBB/3143R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Ubiquitin B Antibody (UBB/3143R) - Azide and BSA Free [NBP3-08907] - Formalin-fixed, paraffin-embedded human Brain stained with Ubiquitin B Recombinant Rabbit Monoclonal Antibody (UBB/3143R).

Western Blot Analysis of Raji lysate using Ubiquitin B Antibody (UBB/3143R) - Azide and BSA Free

• Reactivity: Hu
• Applications: IHC
• Clone: UBB/3143R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Ubiquitin B Antibody (UBB/3143R) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07165).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Ubiquitin B protein (around aa 1-119) (exact sequence is proprietary)
• Localization: Cell Surface, Cytoplasmic and Nuclear
• Marker: Autophagy Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
• Theoretical MW: 9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ubiquitin B Antibody (UBB/3143R) - Azide and BSA Free

• FLJ25987
• MGC8385
• polyubiquitin B
• polyubiquitin-B
• RPS27A
• UBA52
• UBC
• ubiquitin B
• Ubiquitin+1

Background

ubiquitin B encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBB