UBA52 Antibody - BSA Free

Western Blot: UBA52 Antibody [NB300-263] - Detection of Ubiquitin by Western Blotting of Products from an Ubiquitination Assay and Purified Ubiquitin. Samples: A) Ubiquitination assay was performed as described in Zhang et al., 2004, Mol. Cell. Biol. 24(24):10941-10953. B) Purified human ubiquitin was applied in decreasing amounts from 5 to 0.1 mcg. Antibody: Affinity purified rabbit anti-Ubiquitin antibody NB 300-263 used at 0.4 mcg/ml for Western Blot. Detection: Alkaline phosphatase conjugated secondary antibody and development with NBT/BCIP for 10 minutes (A and B).

Immunohistochemistry: UBA52 Antibody [NB300-263] - Sample: FFPE sections of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- Ubiquitin used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

UBA52 Antibody - BSA Free Summary

• Immunogen: The immunogen this antibody was made to, maps to a region between residues 1 and 50 of human Ubiquitin using the numbering given in entry P62988.(GeneID 7311).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: UBA52
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Western Blot 1:1000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for UBA52 Antibody - BSA Free

• CEP52ubiquitin carboxyl extension protein 52
• HUBCEP52
• MGC126879
• MGC126881
• MGC57125
• RPL40
• UBCEP2
• ubiquitin A-52 residue ribosomal protein fusion product 160S ribosomal protein L40
• ubiquitin-52 amino acid fusion protein
• ubiquitin-60S ribosomal protein L40
• ubiquitin-CEP52

Background

Ubiquitin (Ub) is a small 76aa highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins provides an important means to regulate protein abundance and activity by tagging them for degradation. Ubiquitination is a highly regulated and reversible process that appears to be involved in the regulation of many cellular processes and a variety of clinical disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for UBA52 Antibody (NB300-263)(1)

Publication using NB300-263

• Zhang P, Wang C, Gao K et al. The ubiquitin ligase itch regulates apoptosis by targeting thioredoxin-interacting protein for ubiquitin-dependent degradation. J Biol Chem 2010-03-01 [PMID: 20068034]

Bioinformatics

• Gene Symbol: UBA52