Ubiquitin Antibody (1002A) [Unconjugated]

Images

 
Western blot shows Di-Ubiquitin chains of the indicated linkage type loaded at 5 ng/lane. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # MAB8595) followed by ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated with MG132. PVDF membrane was probed with 0.1 μg/mL of Rabbit Anti-Human Ubiquitin Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
1002A
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Ubiquitin Antibody (1002A) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
HEK293 human embryonic kidney cell line transfected with human Ubiquitin
Accession # P0CG47
Specificity
Detects human Ubiquitin in direct ELISAs
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
UBB
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ubiquitin Antibody (1002A) [Unconjugated]

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC
  • Ubiquitin

Background

Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalently attached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has a major role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Ubiquitin Antibody (MAB8595) (0)

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Secondary Antibodies

 

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Ubiquitin-Mediated Degradation of Cellular Proteins: The Kiss of Death
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Bioinformatics

Gene Symbol UBB
Uniprot