u-Plasminogen Activator/Urokinase Antibody (JM106-09)

Western Blot: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of Urokinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-3 cell lysateLane 2: MCF-7 cell lysate

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded mouse kidney tissue using anti-Urokinase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded human colon carcinoma tissue using anti-Urokinase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded human kidney tissue using anti-Urokinase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: u-Plasminogen Activator/Urokinase Antibody (JM106-09) [NBP2-66766] - Analysis of paraffin-embedded human liver carcinoma tissue using anti-Urokinase antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: JM106-09
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

u-Plasminogen Activator/Urokinase Antibody (JM106-09) Summary

• Description: Novus Biologicals Rabbit u-Plasminogen Activator/Urokinase Antibody (JM106-09) (NBP2-66766) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human u-Plasminogen Activator/Urokinase aa 50-99 / 431. (SwissProt: P00749 Human; SwissProt: P06869 Mouse; SwissProt: P29598 Rat)
• Localization: Secreted.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PLAU
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for u-Plasminogen Activator/Urokinase Antibody (JM106-09)

• ATF
• EC 3.4.21
• EC 3.4.21.73
• plasminogen activator, urokinase
• PLAU
• uPA
• u-PA
• uPlasminogen Activator
• u-Plasminogen Activator
• urinary
• Urokinase
• urokinase-type plasminogen activator

Background

Urokinase specifically cleave the zymogen plasminogen to form the active enzyme plasmin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLAU