u-Plasminogen Activator/Urokinase Antibodies

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="u-Plasminogen Activator (uPA)/Urokinase was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human u-Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections." class="big_thumb" />

1310-SE) was serially diluted and captured by Mouse Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Catalytic Domain Monoclonal Antibody (Catalog # MAB1310) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1310-SE) was serially diluted and captured by Mouse Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Catalytic Domain Monoclonal Antibody (Catalog # MAB1310) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1310-SE) was serially diluted and captured by Mouse Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Catalytic Domain Monoclonal Antibody (Catalog # MAB1310) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1310-SE) was serially diluted and captured by Mouse Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Catalytic Domain Monoclonal Antibody (Catalog # MAB1310) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (Catalog # 1310-SE) was serially diluted and captured by Mouse Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Catalytic Domain Monoclonal Antibody (Catalog # MAB1310) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human u‑Plasminogen Activator (uPA)/Urokinase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1310) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF005). A specific band was detected for u-Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for u-Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse u-Plasminogen Activator (uPA)/Urokinase Monoclonal Antibody (Catalog # MAB9185) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for u-Plasminogen Activator (uPA)/Urokinase at approximately 54 and 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />