Tyrosine Hydroxylase Antibody (2D3.1G7)

Images

 
Immunohistochemistry: Tyrosine Hydroxylase Antibody (2D3.1G7) [NBP2-42212] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain /substantia nigra using Tyrosine Hydroxylase antibody (clone ...read more
Immunocytochemistry/ Immunofluorescence: Tyrosine Hydroxylase Antibody (2D3.1G7) [NBP2-42212] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% ...read more
Western Blot: Tyrosine Hydroxylase Antibody (2D3.1G7) [NBP2-42212] - WB analysis of a partial - recombinant human Tyrosine Hydroxylase protein and a lysate of HEK293 cells using 3 ug/ml concentration of Tyrosine ...read more
Flow Cytometry: Tyrosine Hydroxylase Antibody (2D3.1G7) [NBP2-42212] - An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (2D3.1G7) antibody NBP2-42212AF488 (blue) and a matched isotype ...read more
Flow Cytometry: Tyrosine Hydroxylase Antibody (2D3.1G7) [NBP2-42212] - Using the PE direct conjugate An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (2D3.1G7) antibody NBP2-42212PE (blue) ...read more
Flow (Intracellular): Tyrosine Hydroxylase Antibody (2D3.1G7) [NBP2-42212] - An intracellular stain was performed on SH-SY5Y cells with Tyrosine Hydroxylase (2D3.1G7) antibody NBP2-42212 (blue) and a matched isotype ...read more

Product Details

Summary
Reactivity Hu, Rt, Po, Ca, ChHa, RbSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clone
2D3.1G7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Tyrosine Hydroxylase Antibody (2D3.1G7) Summary

Immunogen
Partial recombinant human Tyrosine Hydroxylase protein made to a C-terminal sequence (between amino acids 300-528) [UniProt P07101]
Localization
Cytoplasm, Membrane, Nucleus
Predicted Species
Porcine (92%), Rabbit (90%), Chinese Hamster (91%), Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TH
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 3 ug/ml
  • Flow Cytometry 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 5-15 ug/ml
  • Immunohistochemistry-Paraffin 5-15 ug/ml
  • Flow (Intracellular)
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Brain Hippocampus Lysate (NB820-59187)
Brain Lysate (NB820-59177)
Tyrosine Hydroxylase Lysate (NBP2-11149)
Publications
Read Publication using NBP2-42212.

Reactivity Notes

Immunogen's sequence similarity with other species: Rat (88%), Bovine (88%), Chicken (69%), Xenopus (68%), Zebrafish (64%).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Tyrosine Hydroxylase Antibody (2D3.1G7)

  • DYT14
  • DYT5b
  • EC 1.14.16
  • EC 1.14.16.2
  • TH
  • TYH dystonia 14
  • TYH
  • Tyrosine 3-hydroxylase
  • tyrosine 3-monooxygenase
  • Tyrosine Hydroxylase

Background

Tyrosine hydroxylase (TH) is a rate-limiting enzyme for catecholamine biosynthesis. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia, affective disorders, and Parkinsonism (1). Catecholamines that are produced by the catecholamine biosynthetic pathway, in which tyrosine hydroxylase catalyzes the initial, rate-limiting step, include dopamine, noradrenaline, and adrenaline. These 3 catecholamines are important neurotransmitters and hormones that regulate visceral functions, motor coordination, and arousal in adults. The TH gene becomes transcriptionally active in developing neuroblasts during midgestation of rodent embryos, before the onset of neurotransmission (2). Tyrosine hydroxylase catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-DOPA), the rate-limiting step in the biosynthesis of dopamine (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tyrosine Hydroxylase Antibody (NBP2-42212)(1)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tyrosine Hydroxylase Antibody (NBP2-42212) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-42212AF405
Alexa Fluor 488 Labeled NBP2-42212AF488
Alexa Fluor 647 Labeled NBP2-42212AF647
Alexa Fluor 700 Labeled NBP2-42212AF700
Allophycocyanin Labeled NBP2-42212APC
Biotin Labeled NBP2-42212B
DyLight 350 Labeled NBP2-42212UV
DyLight 405 Labeled NBP2-42212V
DyLight 488 Labeled NBP2-42212G
DyLight 550 Labeled NBP2-42212R
DyLight 650 Labeled NBP2-42212C
DyLight 680 Labeled NBP2-42212FR
DyLight 755 Labeled NBP2-42212IR
HRP Labeled NBP2-42212H
PE Labeled NBP2-42212PE
PerCP Labeled NBP2-42212PCP

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Bioinformatics Tool for Tyrosine Hydroxylase Antibody (NBP2-42212)

Discover related pathways, diseases and genes to Tyrosine Hydroxylase Antibody (NBP2-42212). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Tyrosine Hydroxylase Antibody (NBP2-42212)

Discover more about diseases related to Tyrosine Hydroxylase Antibody (NBP2-42212).
 

Pathways for Tyrosine Hydroxylase Antibody (NBP2-42212)

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PTMs for Tyrosine Hydroxylase Antibody (NBP2-42212)

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Research Areas for Tyrosine Hydroxylase Antibody (NBP2-42212)

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Blogs on Tyrosine Hydroxylase.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Tyrosine hydroxylase - a marker for dopaminergic neurons in the central nervous system
Tyrosine hydroxylase is a member of the aromatic amino acid hydroxylase (AAAH) family.  It is expressed throughout the central nervous system (CNS) and catalyzes the conversion of tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), which can be, thr...  Read full blog post.

Tyrosine Hydroxylase - rate-limiting enzyme in catecholamine synthesis
Catecholamines are tyrosine-derived hormones that are produced in the adrenal gland. They include epinephrine, norepinephrine, and dopamine and are used as neurotransmitters by the central and peripheral nervous system. The rate limiting enzyme in...  Read full blog post.

A Big Guy for the Catecholamine Synthesis - Tyrosine hydroxylase (TH)
In the synthesis pathway for the catecholamines - dopamine, epinephrine, and norepinephrine, tyrosine hydroxylase is the rate-limiting enzyme. Through alternative mRNA splicing, a wide molecular diversity of TH isoforms are generated that are tissue-s...  Read full blog post.

Tyrosine Hydroxylase Deficiencies and Neurodegeneration
Tyrosine hydroxylase is the rate-limiting enzyme in the synthesis pathway of the catecholamines dopamine, epinephrine, and norepinephrine. Alternative mRNA splicing generates a wide molecular diversity of TH isoforms that are tissue specific and produ...  Read full blog post.

Tyrosine Hydroxylase Deficiency and Brain Disorders
 Tyrosine hydroxylase catalyzes the rate-limiting step in the biosynthesis of the catecholamines dopamine, norepinephrine, and epinephrine. A hallmark of Parkinson's disease is the loss of dopaminergic neurons in the substantia nigra. Mutations in cas...  Read full blog post.

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Bioinformatics

Gene Symbol TH
Entrez
Uniprot