Twist-2 Antibody

Twist-2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Twist-2 Antibody Summary

• Immunogen: E. coli-derived recombinant human Twist-2
  Met1-Ser159
  Accession # Q8WVJ9
• Specificity: Detects human Twist-2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Twist-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TWIST2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Twist-2 Antibody

• bHLHa39
• bHLHa39twist-related bHLH protein Dermo1
• Class A basic helix-loop-helix protein 39
• Dermis-expressed protein 1
• DERMO1
• Dermo-1
• DERMO1MGC117334
• twist homolog 2 (Drosophila)
• Twist2
• Twist-2
• twist-related protein 2

Background

Twist-2 (Twist-related protein 2; also Dermo-1 and bHLHa39) is a 19-20 kDa Class B member of the bHLH transcription factor family of proteins. It is expressed in embryonic mesodermal and ectodermal derivatives, and is widely expressed in adult cells, including preosteoblasts, gastric chief cells, preadipocytes and myeloid progenitor cells. Twist-2 is a transcriptional repressor that heterodimerizes with multiple partners such as E12, SREBP1 and MEF2. Human Twist-2 is 160 amino acids (aa) in length. It contains a bHLH domain (aa 65-122) with an embedded DNA-binding motif. Full length human Twist-2 shares 82% and 99% aa identity with human Twist-1 and mouse Twist-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Twist-2 Antibody (AF6249)(2)

Publications using AF6249

• Maria-Vittoria Sepporta, Viviane Praz, Katia Balmas Bourloud, Jean-Marc Joseph, Nicolas Jauquier, Nicolò Riggi et al. TWIST1 expression is associated with high-risk neuroblastoma and promotes primary and metastatic tumor growth Communications Biology 2022-01-12 [PMID: 35022561]
• Jokerst JV, Chen Z, Xu L et al. A Magnetic Bead-Based Sensor for the Quantification of Multiple Prostate Cancer Biomarkers. PLoS One 1/1/2015 [PMID: 26421725]

Bioinformatics

• Gene Symbol: TWIST2
• Uniprot:
  • Q8WVJ9(Human)