Human Twist-1 ELISA Kit (Colorimetric)

ELISA: Human Twist-1 ELISA Kit (Colorimetric) [NBP3-06809] - Samples were spiked with high concentrations of Human Twist-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Twist-1 ELISA Kit (Colorimetric) [NBP3-06809] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL

Human Twist-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Twist-1 in samples. No significant cross-reactivity or interference between Human Twist-1 and analogues was observed.
• Standard Curve Range: 62.5 - 4000 pg/mL
• Sensitivity: 37.5 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 5.46
• Intra-Assay: 5.31
• Spike Recovery: 88 - 107 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TWIST1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Twist-1 ELISA Kit (Colorimetric)

• acrocephalosyndactyly 3
• ACS3
• B-HLH DNA binding protein
• BHLHA38
• bHLHa38BPES3
• BPES3
• Class A basic helix-loop-helix protein 38
• CRS1
• H-twist
• H-twistblepharophimosis, epicanthus inversus and ptosis 3
• SCSBPES2
• twist homolog 1 (Drosophila)
• TWIST homolog of drosophila
• TWIST
• Twist1
• Twist-1
• twist-related protein 1

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Twist-1 ELISA Kit (NBP3-06809)(1)

Publication using NBP3-06809

• Tao M, Zheng D, Liang X et al. Tripterygium glycoside suppresses epithelial to mesenchymal transition of diabetic kidney disease podocytes by targeting autophagy through the mTOR/Twist1 pathway Molecular medicine reports 2021-08-01 [PMID: 34165172] (ELISA)

FAQs for Twist-1 ELISA Kit (NBP3-06809). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TWIST1