TUBA6 [p Ser165] Antibody

Western Blot: TUBA6 [p Ser165] Antibody [NBP3-23260] - Western blot analysis of 250 ng/lane of a1-Tubulin unphosphorylated (lanes 1, 3, & 5) or phosphorylated at Ser-165 with PKC alpha (lanes 2, 4, & 6). The blots were probed with anti-TUBA6 (a.a. 160-169) (NBP3-23259; lanes 1 & 2), anti-TUBA6 (Ser-165) (NBP3-23260; lanes 3 & 4), and anti-alpha Tubulin (C-terminus) (NBP3-23258; lanes 5 & 6).

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TUBA6 [p Ser165] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-TUBA6 (Ser-165) synthetic peptide (coupled to KLH) corresponds to amino acid residues around serine 165 of human TUBA6. This sequence is well conserved in most alpha-tubulin isoforms from most eukaryotic species, but is not conserved in beta-Tubulin. [UniProt# Q9BQE3]
• Modification: p Ser165
• Specificity: This antibody was cross-adsorbed to unphosphorylated TUBA6 (a.a. 160-169) peptide before affinity purification using TUBA6 (Ser-165) peptide (without carrier). The antibody detects a 50 kDa protein corresponding to the molecular mass of a-Tubulin on SDS-PAGE immunoblots of recombinant a1-tubulin phosphorylated with PKC alpha. This reactivity is not observed after lambda phosphatase treatment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBA1C
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for TUBA6 [p Ser165] Antibody

• Alpha-tubulin 6
• bcm948
• MGC10851
• MGC14580
• TUBA1C
• TUBA6
• tubulin alpha-1C chain
• Tubulin alpha-6 chain
• tubulin, alpha 1c
• tubulin, alpha 6

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBA1C