TTK Antibody (689913) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clone: 689913
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

TTK Antibody (689913) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human TTK
  Ser281-Leu481
  Accession # P33981
• Specificity: Detects human TTK in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CaM Kinase II, rhCAM Kinase II alpha , rhCaM Kinase II beta , rhCaM Kinase IIδ, rhCaM Kinase II gamma , rhCaM Kinase III, rhCaM Kinase IV, rhCaM Kinase
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TTK Antibody (689913) [Alexa Fluor® 750]

• CT96
• dual specificity protein kinase TTK
• EC 2.7.12.1
• ESK
• FLJ38280
• monopolar spindle 1-like 1
• Mps1
• MPS1L1
• MPS1L1PYTcancer/testis antigen 96
• phosphotyrosine picked threonine kinase
• Phosphotyrosine picked threonine-protein kinase
• PYT
• TTK protein kinase
• TTK

Background

TTK (Dual specificity Thr/Tyr kinase; also PYT and hMps1) is a 97-105 kDa member of the Ser/Thr protein kinase family of enzymes. It is expressed in testis and thymus, and participates in cell cycle regulation. In particular, it directs chromosome alignment, centrosome duplication, and spindle assembly checkpoint formation. Via phosphorylation, TTK is known to regulate p53, CDCA8 and TACC2. Human TTK is 857 amino acids (aa) in length. It contains one protein kinase domain (aa 525‑791). Activation occurs following homodimerization and autophosphorylation on Thr675, Thr676 and Thr686. There are two potential splice variants. One shows an alternative start site at Met17, while another shows a deletion of Gln420. Over aa 281-481, human TTK shares 53% aa identity with mouse TTK.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.