TTC11 Antibody

Images

 
Genetic Strategies: Western Blot: TTC11 Antibody [NB100-56646] - Analysis of Fis1 in A) human HeLa and B) mouse NIH 3T3 lysate using Fis1 antibody at 1:1000 dilution. B. Specificity validation of the Fis1 ...read more
Immunohistochemistry-Paraffin: TTC11 Antibody [NB100-56646] - Staining of human liver tissue at 1:100.
Immunohistochemistry-Paraffin: TTC11 Antibody [NB100-56646] - Analysis of methcarn-fixed, paraffin-embedded hippocampus from control (normal) and Alzheimer brain. Quantification in five AD brains and five age-matched ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

TTC11 Antibody Summary

Immunogen
Amino acids 11-25 of human Fis1 protein were used as the immunogen.
Predicted Species
Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FIS1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Immunohistochemistry (paraffin): See Wang et al, 2009 for details. Use in ELISA reported in scientific literature (PMID 27494529).
Control
NIH 3T3 Whole Cell Lysate
TTC11 Overexpression Lysate
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-56646 in the following applications:

Reactivity Notes

Predicted to react with Chimpanzee (66%), Canine (93%).Rat and mouse reactivity reported in scientific literature.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera with PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for TTC11 Antibody

  • CGI-135
  • FIS1 homolog
  • Fis1
  • fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
  • fission 1 (mitochondrial outer membrane) homolog (yeast)
  • H_NH0132A01.6
  • hFis1
  • mitochondrial fission 1 protein
  • tetratricopeptide repeat domain 11
  • Tetratricopeptide repeat protein 11
  • TTC11TPR repeat protein 11

Background

TTC11 is a 17KDa integral membrane protein, and is a novel member of the mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Ca
Applications: WB, ELISA, IHC, IHC-P
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Publications for TTC11 Antibody (NB100-56646)(17)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ELISA, IHC-P, WB.


Filter By Application
ELISA
(1)
IHC-P
(1)
WB
(12)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
Rat
(4)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-56646 Applications Species
Abid H, Ryan ZC, Delmotte P et al. Extramyocellular interleukin-6 influences skeletal muscle mitochondrial physiology through canonical JAK/STAT signaling pathways FASEB J. Sep 3 2020 [PMID: 32885495]
Brown JL, Rosa-Caldwell ME, Lee DE et al. Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice J Cachexia Sarcopenia Muscle. 2017 Dec 01 [PMID: 28845591] (WB, Mouse) WB Mouse
El-Sikhry HE, Alsaleh N, Dakarapu R et al. Novel Roles of Epoxyeicosanoids in Regulating Cardiac Mitochondria. PLoS ONE. Aug 6 2016 [PMID: 27494529] (WB, ELISA) WB, ELISA
Differential Mitochondrial Adaptation in Primary Vascular Smooth Muscle Cells from a Diabetic Rat Model. Keller AC, Knaub LA, McClatchey PM Oxid Med Cell Longev [PMID: 27034743] (WB, Rat) WB Rat
Placido AI, Pereira CM, Correira SC et al. Phosphatase 2A Inhibition Affects Endoplasmic Reticulum and Mitochondria Homeostasis Via Cytoskeletal Alterations in Brain Endothelial Cells. Mol. Neurobiol. 2016 Jan 05 [PMID: 26732598] (WB, Rat)

Details:
Fis1/TTC11 antibody was used for WB assays on lysates from rat brain endothelial cell line/RBE4 cells which were treated or not with taxol, nocadazole or cytochalasin B.
WB Rat
Santos D, Esteves AR, Silva DF et al. The Impact of Mitochondrial Fusion and Fission Modulation in Sporadic Parkinson's Disease. Mol Neurobiol. 2014 Sep 14 [PMID: 25218511] (WB, Human)

Details:
Fis1 /TTC11 antibody used for WB on cytosolic and mitochondrial preparations from CT and PD (sporadic Parkinson’s disease ) cybrids - the cytoplasmic hybrid cells prepared via the transfer of PD subject platelet mitochondria to mitochondrial DNA /mtDNA-depleted (rho0) human NT2 teratocarcinoma cells. Cybrids represents a model in which mitochondrial platelet mitochondria CXI defect persists and mitochondrial dysfunction is well characterized. Antibody also used for WB on PBMCs isolated from PD patients.
WB Human
Santos RX, Correia SC, Alves MG et al. Mitochondrial quality control systems sustain brain mitochondrial bioenergetics in early stages of type 2 diabetes. Mol. Cell. Biochem. 2014 May 16 [PMID: 24833464] (WB, Rat)

Details:
Rat brain cortical tissue, Fig 2A.The Fis1 pAb was used at 1:750 and Fis1 was detected at ~17 kDa.
WB Rat
Santos RX, Xcorreia SC, Alves MG et al. Insulin therapy modulates mitochondrial dynamics and biogenesis, autophagy and tau protein phosphorylation in the brain of type 1 diabetic animals. Biochim. Biophys. Acta 2014 Apr 18 [PMID: 24747740] (WB, Rat)

Details:
Mitochondrial extracts of brain cortex, Fig 3A. Fis1 was detected at ~17kDa.
WB Rat
Liu R, Jin P, Liqunyu et al. Impaired mitochondrial dynamics and bioenergetics in diabetic skeletal muscle. PLoS ONE 2014 Mar 24 [PMID: 24658162] (WB, Mouse)

Details:
Skeletal muscle, Fig 7
WB Mouse
Show All 17 Publications.

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Bioinformatics

Gene Symbol FIS1
Entrez
Uniprot