TPR Antibody

Images

 
Western Blot: TPR Antibody [NB100-2866] - Whole cell lysate from HeLa (5, 15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TPR antibody used for WB at 0.04 ug/ml. Detection: ...read more
Immunocytochemistry/ Immunofluorescence: TPR Antibody [NB100-2866] - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue). Image submitted by a verified customer review.
Independent Antibodies: Western Blot: TPR Antibody [NB100-2866] - Detection of Human TPR on HeLa whole cell lysate using NB100-2866. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, ...read more
Independent Antibodies: Knockdown Validated: TPR Antibody [NB100-2866] - Lane 1 contians wildtype MDA-MB-231 cell lysate nuclear extract. Lanes 2, 3 and 4 contain the TPR knocked down MDA-MB-231 cell lysate ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

TPR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2275 and 2325 of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 24344181) and customer review. TPR antibody validated for KD from a verified customer review.
Theoretical MW
267 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-2866 in the following applications:

Publications
Read Publications using
NB100-2866 in the following applications:

  • 1 publication
  • KD
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TPR Antibody

  • nuclear pore complex-associated protein TPR
  • nucleoprotein TPR
  • translocated promoter region (to activated MET oncogene)
  • tumor potentiating region

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TPR Antibody (NB100-2866)(4)

Reviews for TPR Antibody (NB100-2866) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-2866:
Filter by Applications
Western Blot
(1)
Immunofluorescence
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot TPR NB100-2866
Enlarge
5
reviewed by:
David Feuersinger
Western Blot Human 01/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedMDA-MB-231 Cell Lysate nuclear Extract,MDA-MB-231 Cell Lysate nuclear extract
SpeciesHuman
LotA1

Comments

CommentsDilution 1:5000
Immunofluorescence TPR NB100-2866
Enlarge
5
reviewed by:
Cristina Ferrás
Immunofluorescence Human 08/27/2014
View

Summary

ApplicationImmunofluorescence
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPR Antibody (NB100-2866) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TPR Products

Array NB100-2866

Bioinformatics Tool for TPR Antibody (NB100-2866)

Discover related pathways, diseases and genes to TPR Antibody (NB100-2866). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TPR Antibody (NB100-2866)

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Pathways for TPR Antibody (NB100-2866)

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Blogs on TPR

There are no specific blogs for TPR, but you can read our latest blog posts.
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Contact Information

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Calculators

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

David Feuersinger
01/24/2018
Application: Western Blot
Species: Human

Cristina Ferrás
08/27/2014
Application: Immunofluorescence
Species: Human

Bioinformatics

Gene Symbol TPR
Entrez
Uniprot