TPR Antibody

Images

 
Western Blot: TPR Antibody [NB100-2866] - Detection of Human TPR on HeLa whole cell lysate using NB100-2866. TPR was also immunoprecipitated by rabbit anti-TPR antibodies NB100-2864, NB100-2865 and NB100-2867.
Proximity Ligation Assay: TPR Antibody [NB100-2866] - TPR and Mad1 interaction at the Nuclear Pores (green dots). DNA counterstained with DAPI (blue). Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

TPR Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 2275 and 2325 of human Translocated Promoter Region Protein using the numbering given in entry NP_003283.1 (GeneID 7175).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24344181). PLA data from customer review.
Theoretical MW
267 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-2866 in the following applications:

Publications
Read Publication using
NB100-2866 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TPR Antibody

  • nuclear pore complex-associated protein TPR
  • nucleoprotein TPR
  • translocated promoter region (to activated MET oncogene)
  • tumor potentiating region

Background

This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5' end of this gene with several different kinase genes occur in some neoplasias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TPR Antibody (NB100-2866)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(1)
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Review for TPR Antibody (NB100-2866) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NB100-2866:
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IF
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence TPR NB100-2866
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reviewed by:
Anonymous
IF Human 08/27/2014
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Summary

ApplicationImmunofluorescence
SpeciesHuman
Special ApplicationsProximity Ligation Assay

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TPR Antibody (NB100-2866) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TPR Antibody (NB100-2866)

Discover related pathways, diseases and genes to TPR Antibody (NB100-2866). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TPR Antibody (NB100-2866)

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Pathways for TPR Antibody (NB100-2866)

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Blogs on TPR

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Recent Reviews

5
5
1
4
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0
2
0
1
0

Anonymous
08/27/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol TPR
Entrez
Uniprot