TSPAN1 Antibody (819202) [Unconjugated]

HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with either (A) human TSPAN1 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSPAN1 Monoclonal Antibody (Catalog # MAB9186) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB007). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 819202
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TSPAN1 Antibody (819202) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TSPAN1
  Met1-Gln241
  Accession # O60635
• Specificity: Detects human TSPAN1 in direct ELISAs.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSPAN1 Antibody (819202) [Unconjugated]

• NET1
• NET-1
• tetraspan 1
• Tetraspan NET-1
• tetraspanin 1
• Tetraspanin TM4-C
• tetraspanin-1
• TM4C
• TM4SF
• TSPAN1
• TSPAN-1

Background

Tetraspanins (TSPAN family) are small integral proteins that contain four transmembrane domains with two different and well characterized extracellular domains. Tetraspanins are found on the plasma membrane and within various intracellular organelles and granules in nearly all cell and tissue types. They interact with one another and with other transmembrane molecules, including integrins, generating a ‘web' of interactions believed to control cell and membrane trafficking and compartmentalization. Tetraspanin-1 (TSPAN1), a 241 amino acid protein, interacts with the thiamine transporter-1 (THTR-1, SLC19A2) in human intestinal epithelial cells, linking TSPAN1 to the regulation of thiamine uptake. TSPAN1 has been found to be expressed in gastric, ovarian, colon, and other carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O60635(Human)