TSLP Antibody

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Biological Strategies: Western Blot: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/Nga mice. Lysates were immunoblotted with ...read more
Immunocytochemistry/ Immunofluorescence: TSLP Antibody [NB110-55234] - Staining of Mouse brain tissue with antibody at 20 ug/ml.
Immunohistochemistry: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/ Nga mice. Paraffin sections of skin biopsies from DNCB-induced AD-like ...read more
Western Blot: TSLP Antibody [NB110-55234] - A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via ...read more
Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2).Loading: 15 ug of lysates per lane. Antibodies: TSLP NB110-55234 (4 ug/mL), 1h incubation at RT in ...read more
Expression of Tagged Proteins: Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30ng of TSLP partial human recombinant protein per lane. ...read more
Immunocytochemistry/ Immunofluorescence: TSLP Antibody [NB110-55234] - Human Brain cells.
Immunohistochemistry-Paraffin: TSLP Antibody [NB110-55234] - Human brain tissue with TSLP antibody at 2.5 ug/mL.
Immunohistochemistry-Paraffin: TSLP Antibody [NB110-55234] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

TSLP Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 19 amino acids near the center of human TSLP. The immunogen is located within the amino acids 40-90 of TSLP. Amino Acid Sequence: SGTKSTEFNNTVSCSNRPH
Specificity
Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD). NB110-55234 can detect only longer human isoform, but cannot detect shorter human isoform, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSLP
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25-4 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Immunofluorescence 20 ug/mL
Application Notes
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human and mouse samples; Immunocytochemistry in human samples. All other applications and species not yet tested. Predicted: 18 kD; Observed: 17, 24 kD (Post-modifications: 2 N linked glycosylations)
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TSLP Peptide (NB110-55234PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB110-55234 in the following applications:

Publications
Read Publications using
NB110-55234 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Peptide affinity purified

Alternate Names for TSLP Antibody

  • thymic stromal lymphopoietin
  • TSLP

Background

Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TSLP Antibody (NB110-55234)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
WB
(3)
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Filter By Species
Human
(1)
Mouse
(3)
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Showing Publications 1 - 5 of 5.
Publications using NB110-55234 Applications Species
Kim YJ, Choi MJ, Bak DH et al. Topical administration of EGF suppresses immune response and protects skin barrier in DNCB-induced atopic dermatitis in NC/Nga mice Sci Rep Aug 9 2018 [PMID: 30093649] (WB, Mouse) WB Mouse
Buchheit KM, Cahill KN, Katz HR et al. Thymic stromal lymphopoietin controls prostaglandin D2 generation in patients with aspirin-exacerbated respiratory disease. J. Allergy Clin. Immunol. 2015 Dec 12 [PMID: 26691435]

Details:
TSLP antibody was used for WB analysis of lysates from nasal polyp samples of patients with aspirin-exacerbated respiratory disease/AERD (Fig 4; Fig E3). The antibody was tested for specificity using its corresponding blocking peptide NB110-55234PEP and was also compared with anti-TSLP antibody (ABT330 from Millipore; Fig E2).
Sziksz E, Molnar K, Lippai R et al. Peroxisome proliferator-activated receptor-y and thymic stromal lymphopoietin are involved in the pathophysiology of childhood coeliac disease. Virchows Arch. 2014 Sep 04 [PMID: 25187315] (IHC-P, Human) IHC-P Human
Han Sang-Chul, Kang Gyeoung-Jin, Ko Yeong-Jong et al. External Application of Fermented Olive Flounder (Paralichthys olivaceus) Oil Alleviates Inflammatory Responses in 2,4-Dinitrochlorobenzeneinduced Atopic Dermatitis Mouse Model. Toxicological Research 2012 [PMID: 24278605] (IHC, WB, Mouse) IHC, WB Mouse
Han Sang-Chul, Kang Gyeoung-Jin, Ko Yeong-Jong et al. Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via geneRation of CD4+CD25+Foxp3+ T cells. Bmc Immunology 2012 [PMID: 22873180] (IHC, WB, Mouse) IHC, WB Mouse

Review for TSLP Antibody (NB110-55234) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-55234:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot TSLP NB110-55234
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5
reviewed by:
Verified Customer
WB Human 07/01/2017
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Summary

ApplicationWestern Blot
Sample TestedNasal tissue
SpeciesHuman

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ICC/IF Video Protocol

FAQs for TSLP Antibody (NB110-55234) (0)

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Recent Reviews

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5
1
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1
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Verified Customer
07/01/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol TSLP
Entrez
Uniprot