Biological Strategies: Western Blot: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/Nga mice. Lysates were immunoblotted with ...read more
Immunocytochemistry/ Immunofluorescence: TSLP Antibody [NB110-55234] - Staining of Mouse brain tissue with antibody at 20 ug/ml.
Immunohistochemistry: TSLP Antibody [NB110-55234] - EGF attenuates protein expression of PAR-2 and TSLP in DNCB-induced AD-like skin lesions in NC/ Nga mice. Paraffin sections of skin biopsies from DNCB-induced AD-like ...read more
Western Blot: TSLP Antibody [NB110-55234] - A-20 cell lysate with TSLP antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
Western Blot: TSLP Antibody [NB110-55234] - Total protein levels of TSLP in patients with AERD and subjects with CRS measured by using Western blotting of 3 representative samples per group. This image was submitted via ...read more
Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation in Human Heart (Lane 1) and Human Prostate (Lane 2).Loading: 15 ug of lysates per lane. Antibodies: TSLP NB110-55234 (4 ug/mL), 1h incubation at RT in ...read more
Expression of Tagged Proteins: Western Blot: TSLP Antibody [NB110-55234] - Western Blot Validation of TSLP with Human Recombinant protein. Loading: 30ng of TSLP partial human recombinant protein per lane. ...read more
Immunocytochemistry/ Immunofluorescence: TSLP Antibody [NB110-55234] - Human Brain cells.
Immunohistochemistry-Paraffin: TSLP Antibody [NB110-55234] - Human brain tissue with TSLP antibody at 2.5 ug/mL.
Immunohistochemistry-Paraffin: TSLP Antibody [NB110-55234] - Staining of mouse brain tissue with antibody at 2.5 ug/ml.
Antibody was raised against a peptide corresponding to 19 amino acids near the center of human TSLP. The immunogen is located within the amino acids 40-90 of TSLP. Amino Acid Sequence: SGTKSTEFNNTVSCSNRPH
Human TSLP has 2 isoforms, including isoform 1 (159aa, 18kD) and isoform 2 (63aa, 7kD). Mouse TSLP has one isoform (140aa, 16kD) and Rat TSLP also has one isoform (135aa, 16kD). NB110-55234 can detect only longer human isoform, but cannot detect shorter human isoform, mouse and rat.
Peptide affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human and mouse samples; Immunocytochemistry in human samples. All other applications and species not yet tested. Predicted: 18 kD; Observed: 17, 24 kD (Post-modifications: 2 N linked glycosylations)
18 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for TSLP Antibody
thymic stromal lymphopoietin
Thymic stromal lymphopoietin (TSLP) has recently been identified as an important factor capable of driving dendritic cell maturation and activation. TSLP is a four-helix-bundle cytokine that is expressed mainly by barrier epithelial cells and is a potent activator of several cell types such as myeloid dendritic cells. TSLP is involved in the positive selection of regulatory T cells, maintenance of peripheral CD4+ T cell homeostasis and the induction of CD4+ T cell-mediated allergic reaction. TSLP is also capable of supporting the growth of fetal liver and adult B cell progenitors and their differentiation to the IgM-positive stage of B cell development. Amino acid sequence analysis has shown poor homology between human and mouse TSLP although they exhibit similar biological functions and are expressed in similar tissues. Despite its predicted molecular weight, TSLP often migrates at a higher molecular weight in SDS-PAGE. At least two differentially spliced isoforms of TSLP are known to exist.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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