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Tryptophan hydroxylase 2 Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. ...read more
Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan ...read more
Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.
Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot ...read more
Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to ...read more
The 5-HT and TPH2 in the raphe nuclei of the brain. The 5-HT- and TPH2-positive double stained cells in the dorsal raphe nuclei (A). Representative photomicrographs were taken at magnifications of 100×. Quantification ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Tryptophan hydroxylase 2 Antibody - BSA Free Summary

Immunogen
Synthetic peptide make to an internal portion of the mouse Tryptophan hydroxylase 2 protein (between amino acids 10-75) [UniProt Q8CGV2]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry reported in scientific literature (PMID 30581079)
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 26428905)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 30350781)
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Western Blot 1:1000
Application Notes
IF usage was reported in scientific literature (see Soiza-Reillly M et al, 2011).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-74555 in the following applications:

Publications
Read Publications using
NB100-74555 in the following applications:

Reactivity Notes

Human reactivity is weak. Mouse reactivity reported in scientific literature (PMID: 16581041). Rat reactivity reported in scientific literature (PMID: 24066056).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tryptophan hydroxylase 2 Antibody - BSA Free

  • ADHD7
  • EC 1.14.16
  • EC 1.14.16.4
  • FLJ37295
  • MGC138872
  • Neuronal tryptophan hydroxylase
  • NTPH
  • NTPHMGC138871
  • TPH2
  • tryptophan 5-hydroxylase 2
  • Tryptophan 5-monooxygenase 2
  • Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tryptophan hydroxylase 2 Antibody (NB100-74555)(53)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 8 applications: B/N, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, WB.


Filter By Application
B/N
(1)
FLOW
(1)
ICC/IF
(12)
IF/IHC
(15)
IHC
(4)
IHC-Fr
(5)
IHC-FrFl
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(29)
Rat
(2)
Primate
(2)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NB100-74555 Applications Species
Higuchi Y, Tada T, Nakachi T, Arakawa H Serotonergic circuit dysregulation underlying autism-related phenotypes in BTBR mouse model of autism Neuropharmacology 2023-06-09 [PMID: 37301467] (IHC-Fr, Mouse)

Details:
1:800 dilution
IHC-Fr Mouse
Wang J, Mei Y, Zhang X et al. Aberrant serotonergic signaling contributes to the hyperexcitability of CA1 pyramidal neurons in a mouse model of Alzheimer's disease Cell reports 2023-02-22 [PMID: 36821438] (IHC-FrFl, Mouse)

Details:
Dilution used in IHC-FrFl 1:500
IHC-FrFl Mouse
Ganley RP, de Sousa MM, Werder K et al. Targeted anatomical and functional identification of antinociceptive and pronociceptive serotonergic neurons that project to the spinal dorsal horn eLife 2023-02-08 [PMID: 36752606] (IHC, Mouse) IHC Mouse
Jansch C Effects of SLC2A3 copy number variants on neurodevelopment and glucose metabolism in ADHD patient-specific neurons Thesis 2021-01-01 (ICC/IF, Human) ICC/IF Human
Liu S, Xiang K, Yuan F, Xiang M Generation of self-organized autonomic ganglion organoids from fibroblasts iScience 2023-03-01 [PMID: 36922996]
Pinto DFC Convergent Dopamine and Serotonin Reward Signals Govern Associative Learning Thesis 2023-01-01
Li W, Shen Z, Yin X et al. Reduction of p11 in dorsal raphe nucleus serotonergic neurons mediates depression-like behaviors Translational psychiatry 2023-11-22 [PMID: 37993435] (IHC, Mouse)

Details:
1:500 dilution
IHC Mouse
Cheng H, Lou Q, Lai N et al. Projection-defined median raphe Pet+ subpopulations are diversely implicated in seizure Neurobiology of disease 2023-11-15 [PMID: 37977434] (IHC, Mouse)

Details:
1:1000 dilution, Free floating sections
IHC Mouse
Marcinkiewcz C, Wang R, Khan K et al. Alcohol inhibits sociability via serotonin inputs to the nucleus accumbens Research Square 2023-08-18 [PMID: 37461716]
Furukawa M, Tada H, Raju R et al. Long-Term Soft-Food Rearing in Young Mice Alters Brain Function and Mood-Related Behavior Nutrients 2023-05-20 [PMID: 37242280] (IHC, Mouse) IHC Mouse
Show All 53 Publications.

Reviews for Tryptophan hydroxylase 2 Antibody (NB100-74555) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB100-74555:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Tryptophan hydroxylase 2 NB100-74555
Enlarge
5
reviewed by:
Luiz Alexandre Magno
IHC-Fr Mouse 07/19/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmidbrain
SpeciesMouse
Lot1068-102

Comments

CommentsThis is a fantastic antibody to detect TPH2 in free floating slices from PFA-perfused mice. I use it with Alexa Fluor 488 2nd antibody.
  5
reviewed by:
Verified Customer
ICC Mouse 12/27/2011
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse
SpeciesMouse
Immunohistochemistry-Paraffin Tryptophan hydroxylase 2 NB100-74555
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 10/25/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain
SpeciesMouse
LotK1137602

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tryptophan hydroxylase 2 Antibody (NB100-74555) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Luiz Alexandre Magno
07/19/2018
Application: IHC-Fr
Species: Mouse

 
Verified Customer
12/27/2011
Application: ICC
Species: Mouse

Verified Customer
10/25/2010
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol TPH2
Entrez
Uniprot