Tryptophan hydroxylase 2 Antibody - BSA Free

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin. The antibody generated staining of the neurons.

Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Tryptophan Hydroxylase 2 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan hydroxylase 2-positive double stained cells in the dorsal raphe nuclei. Representative photomicrographs were taken at magnifications of 100x. Quantification of 5-HT and Tryptophan hydroxylase 2. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2218-273X/10/1/71), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.

Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot LUT. Image from verified customer review.

Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to raphe nuclei whole cell extracts for 15 minutes at RT. Immunoprecipitated proteins were assessed by Western blotting (WB), using either mouse anti-TPH2 (Ms TPH2, Lane 2) or sheep anti-TPH2 antibodies (Sh TPH2, Lane 3). As a positive control for the Western blot, non-immunoprecipitated brain lysates were probed with the mouse anti-TPH2 antibody (Lane 1). As a specificity control, a blot containing immunoprecipitated proteins was probed with an antibody specific for Tyrosine Hydroxylase (TH, Lane 4). Data courtesy of the Innovators Program.

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Tryptophan hydroxylase 2 Antibody - BSA Free Summary

• Immunogen: Synthetic peptide make to an internal portion of the mouse Tryptophan hydroxylase 2 protein (between amino acids 10-75) [UniProt Q8CGV2]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry Free-Floating 1:10 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Western Blot 1:1000
• Application Notes: IF usage was reported in scientific literature (see Soiza-Reillly M et al, 2011). Use in IHC-FF reported in scientific literature (PMID: 26428905). Use in FLOW reported in scientific literature (PMID: 30581079). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30350781).

Reactivity Notes

Human reactivity is weak. Mouse reactivity reported in scientific literature (PMID: 16581041). Rat reactivity reported in scientific literature (PMID: 24066056).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Tryptophan hydroxylase 2 Antibody - BSA Free

• ADHD7
• EC 1.14.16
• EC 1.14.16.4
• FLJ37295
• MGC138872
• Neuronal tryptophan hydroxylase
• NTPH
• NTPHMGC138871
• TPH2
• tryptophan 5-hydroxylase 2
• Tryptophan 5-monooxygenase 2
• Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tryptophan hydroxylase 2 Antibody (NB100-74555)(43)

Publications using NB100-74555

• Liu S, Xiang K, Yuan F, Xiang M Generation of self-organized autonomic ganglion organoids from fibroblasts iScience 2023-03-01 [PMID: 36922996]
• Wang J, Mei Y, Zhang X et al. Aberrant serotonergic signaling contributes to the hyperexcitability of CA1 pyramidal neurons in a mouse model of Alzheimer's disease Cell reports 2023-02-22 [PMID: 36821438] (IHC-FrFl, Mouse)
  
  Details:
  Dilution used in IHC-FrFl 1:500
• Ganley RP, de Sousa MM, Werder K et al. Targeted anatomical and functional identification of antinociceptive and pronociceptive serotonergic neurons that project to the spinal dorsal horn eLife 2023-02-08 [PMID: 36752606] (IHC, Mouse)
• Huerta-Ocampo I, Dautan D, Gut N et al. Whole-brain mapping of monosynaptic inputs to midbrain cholinergic neurons Sci Rep 2021-04-27
• Jansch C Effects of SLC2A3 copy number variants on neurodevelopment and glucose metabolism in ADHD patient-specific neurons Thesis 2021-01-01 (ICC/IF, Human)
• Pomrenze MB, Cardozo Pinto DF, Neumann PA et al. Modulation of 5-HT release by dynorphin mediates social deficits during opioid withdrawal Neuron 2022-09-29 [PMID: 36202097]
• Luo N, Mosialou I, Capulli M et al. A neuronal action of sirtuin 1 suppresses bone mass in young and aging mice The Journal of clinical investigation 2022-10-04 [PMID: 36194488] (IHC-Fr, Mouse)
• Baytas O, Kauer J, Morrow E Loss of mitochondrial enzyme GPT2 causes early neurodegeneration in locus coeruleus Neurobiology of Disease 2022-07-01 [PMID: 35908744] (IHC-Fr, Mouse)
• Pouchelon G, Dwivedi D, Bollmann Y Et al. The organization and development of cortical interneuron presynaptic circuits are area specific Cell reports 2021-11-09 [PMID: 34758329] (IF/IHC, Mouse)
• Lee JS, Lee SB, Kim DW Et al. Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation Science advances 2021-11-05 [PMID: 34739315] (IHC-Fr, Mouse)
• Ortuno MJ, Schneeberger M, Ilanges A Et al. Melanocortin 4 receptor stimulation prevents anti-depressant-associated weight gain in mice caused by long-term fluoxetine exposure The Journal of clinical investigation 2021-10-21 [PMID: 34673574] (IF/IHC, Mouse)
• Seigneur E, Wang J, Dai J et al. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors Molecular psychiatry 2021-06-22 [PMID: 34158618]
• Vieira JS, de Souza GR, Kalil-Cutti B et al. Post-traumatic stress disorder increases pain sensitivity by reducing descending noradrenergic and serotoninergic modulation Behavioural brain research 2021-05-14 [PMID: 34000338]
• Cheng H, Qi Y, Lai N et al. Inhibition of hyperactivity of the dorsal raphe 5-HTergic neurons ameliorates hippocampal seizure CNS neuroscience & therapeutics 2021-05-06 [PMID: 33955651] (IF/IHC)
• Konig N, Bimpisidis Z, Dumas S, Wallen-Mackenzie A Selective Knockout of the Vesicular Monoamine Transporter 2 (Vmat2) Gene in Calbindin2/Calretinin-Positive Neurons Results in Profound Changes in Behavior and Response to Drugs of Abuse Sci Transl Med 2020-11-25 [PMID: 33240055]
• Okaty BW, Sturrock N, Escobedo Lozoya Y et al. A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons Elife 2020-06-22 [PMID: 32568072] (ICC/IF, Mouse)
• Tudeau L, AcuNa MA, Albisetti GW et al. Mice lacking spinal alpha 2GABAA receptors: Altered GABAergic neurotransmission, diminished GABAergic antihyperalgesia, and potential compensatory mechanisms preventing a hyperalgesic phenotype Brain Res. 2020-05-18 [PMID: 32439345] (IF/IHC, Mouse)
• Dombret C, NaulE L, Trouillet AC et al. Effects of neural estrogen receptor beta deletion on social and mood-related behaviors and underlying mechanisms in male mice Sci Rep 2020-04-10 [PMID: 32277160] (IF/IHC, Mouse)
• Lee JS, Jeon YJ, Park SY, Son CG An Adrenalectomy Mouse Model Reflecting Clinical Features for Chronic Fatigue Syndrome Biomolecules 2020-01-01 [PMID: 31906307] (Mouse)
• Ren J, Isakova A, Friedmann D et al. Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei Elife 2019-10-24 [PMID: 31647409] (ICC/IF, Mouse)
• Li Y, Li CY, Xi W et al. Rostral and Caudal Ventral Tegmental Area GABAergic Inputs to Different Dorsal Raphe Neurons Participate in Opioid Dependence Neuron 2019-02-20 [PMID: 30638902] (IF/IHC, Mouse)
• Thier MC, Hommerding O, Panten J et al. Identification of Embryonic Neural Plate Border Stem Cells and Their Generation by Direct Reprogramming from Adult Human Blood Cells Cell Stem Cell 2018-12-12 [PMID: 30581079] (FLOW, Mouse)
• Dosumu-Johnson RT, Cocoran AE, Chang Y et al. Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery. Elife. 2018-10-23 [PMID: 30350781] (IHC-Fr, Mouse)
• Soiza-Reilly M, Meye FJ, Olusakin J et al. SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior. Mol. Psychiatry. 2018-10-02 [PMID: 30279456] (IF/IHC, Mouse)
• Ehlinger DG, Commons KG. Cav1.2L-type calcium channels regulate stress coping behavior via serotonin neurons. Neuropharmacology 2018-08-31 [PMID: 30176250] (ICC/IF, Mouse)
• Ren J, Friedmann D, Xiong J et al. Anatomically Defined and Functionally Distinct Dorsal Raphe Serotonin Sub-systems Cell 2018-08-18 [PMID: 30146164] (IF/IHC, Mouse)
• Ma J, Wang F, Yang J et al. Xiaochaihutang attenuates depressive/anxiety-like behaviors of social isolation-reared mice by regulating monoaminergic system, neurogenesis and BDNF expression Journal of Ethnopharmacology 2017-08-17 [PMID: 28687505] (Mouse)
• Natarajan R, Forrester L, Chiaia N, Yamamoto BK. Chronic Stress-induced Behavioral Changes Associated with Subregion Selective Serotonin Cell Death in the Dorsal Raphe. J. Neurosci. 2017-05-25 [PMID: 28546314] (ICC/IF, Rat)
• Pomeranz LE, Ekstrand MI, Latcha KN et al. Gene expression profiling with Cre-conditional pseudorabies virus reveals a subset of midbrain neurons that participate in reward circuitry. J. Neurosci. 2017-03-10 [PMID: 28283558] (IF/IHC, Mouse)
• Goto S, Ogi H, Fushiki S, Itoh K. Prenatal and lactational bisphenol A exposure does not alter serotonergic neurons morphologically in the murine dorsal raphe nucleus. Brain Dev. 2017-02-21 [PMID: 28233694]
• Coyle D, Murphy JM, Doyle B et al. Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis. World J Gastroenterol 2016-05-21 [PMID: 27217698] (WB)
• Barrett KT, Dosumu-Johnson RT, Daubenspeck JA et al. Partial Raphe Dysfunction in Neurotransmission Is Sufficient to Increase Mortality after Anoxic Exposures in Mice at a Critical Period in Postnatal Development. J. Neurosci. 2016-04-06 [PMID: 27053202] (ICC/IF, Mouse)
• Commons KG. Ascending serotonin neuron diversity under two umbrellas. Brain Struct Funct. 2016-01-06 [PMID: 26740230] (IF/IHC, Mouse)
• Alter SP, Stout KA, Lohr KM et al. Reduced vesicular monoamine transport disrupts serotonin signaling but does not cause serotonergic degeneration. Exp Neurol 2016-01-01 [PMID: 26428905] (IF/IHC)
• Naslund J, Studer E, Petterson R et al. Differences in anxiety-like behaviour within a batch of Wistar rats are associated with differences in serotonergic transmission, enhanced by acute SSRI administration and abolished by serotonin depletion Int. J. Neuropsychopharmacol. 2015-02-25 [PMID: 25716782] (WB, Rat)
• Stansley BJ, Yamamoto BK. Chronic L-Dopa Decreases Serotonin Neurons in a Sub-region of the Dorsal Raphe Nucleus. J Pharmacol. Exp. Ther. 2014-09-11 [PMID: 25212217]
• Ray RS, Corcoran AE, Brust RD et al. Egr2-neurons control the adult respiratory response to hypercapnia Brain Res 2012-12-19 [PMID: 23261662] (ICC/IF, IF/IHC, Mouse)
• Zhou W, Chen L, Paul J et al. The effects of glycogen synthase kinase-3beta in serotonin neurons PLoS One 2012-01-01 [PMID: 22912839] (IF/IHC, ICC/IF, Mouse)
• Fukada M, Hanai A, Nakayama A, Suzuki T, Miyata N, Rodriguiz RM, Wetsel WC, Yao TP, Kawaguchi Y. Loss of deacetylation activity of hdac6 affects emotional behavior in mice. PLoS One;7(2):e30924. 2012-01-01 [PMID: 22328923]
• Bang SJ, Jensen P, Dymecki SM, Commons KG. Projections and interconnections of genetically defined serotonin neurons in mice Eur J Neurosci 2012-01-01 [PMID: 22151329] (ICC/IF, IF/IHC, Mouse)
• Soiza-Reilly M, Commons KG. Glutamatergic drive of the dorsal raphe nucleus. J Chem Neuroanat 41(4):247-55. doi: 10.1016/j.jchemneu.2011.04.004. 2011-07-01 [PMID: 21550397]
• Tokuyama Y, Ingram SL, Woodward JS et al. Functional characterization of rhesus embryonic stem cell-derived serotonin neurons. Exp Biol Med (Maywood). 2010-05-01 [PMID: 20463306] (ICC/IF, Primate)
• Sullivan EL, Grayson B, Takahashi D et al. Chronic consumption of a high-fat diet during pregnancy causes perturbations in the serotonergic system and increased anxiety-like behavior in nonhuman primate offspring. J Neurosci 2010-03-10 [PMID: 20220017] (ICC/IF, IF/IHC, Primate)

Reviews for Tryptophan hydroxylase 2 Antibody (NB100-74555) (3)

Average Rating: 4.7

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 07/19/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: midbrain
• Species: Mouse
• Lot: 1068-102

Comments

• Comments: This is a fantastic antibody to detect TPH2 in free floating slices from PFA-perfused mice. I use it with Alexa Fluor 488 2nd antibody.

reviewed by: Kathryn Commons ICC Mouse 12/27/2011

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse
• Species: Mouse

reviewed by: Assaf Vestin IHC-P Mouse 10/25/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Brain
• Species: Mouse
• Lot: K1137602

Bioinformatics

• Gene Symbol: TPH2
• Entrez:
  • 121278(Human)
  • 216343(Mouse)
  • 317675(Rat)
• Uniprot:
  • Q8IWU9(Human)