Tryptophan hydroxylase 2 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.
Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Tryptophan hydroxylase 2 Antibody Summary

Immunogen
Synthetic peptide corresponding to the N-terminal region of TPH2: R(15) R G L S L D S A V P E D H Q L (30)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:1000
  • Immunohistochemistry Free-Floating 1:10 - 1:500
Application Notes
IF usage was reported in scientific literature (see Soiza-Reillly M et al, 2011). IHC-P usage was reported by customer review. Use in IHC-FF reported in scientific literature (PMID: 26428905).
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB100-74555 in the following applications:

Publications
Read Publications using
NB100-74555 in the following applications:

  • 7 publications
  • IHC
    7 publications
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 16581041). Rat reactivity reported in scientific literature (PMID: 24066056).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
1.0 mg/ml BSA
Preservative
0.1% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Tryptophan hydroxylase 2 Antibody

  • ADHD7
  • EC 1.14.16
  • EC 1.14.16.4
  • FLJ37295
  • MGC138872
  • Neuronal tryptophan hydroxylase
  • NTPH
  • NTPHMGC138871
  • TPH2
  • tryptophan 5-hydroxylase 2
  • Tryptophan 5-monooxygenase 2
  • Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF5276
Species: Hu
Applications: WB, IHC
H00006532-M06
Species: Hu
Applications: ELISA, IP
NBP2-21590
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-01752
Species: Hu
Applications: WB, IHC, IHC-P
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NBP1-85818
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-26091
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP2-55967
Species: Hu
Applications: ICC/IF
NBP1-31528
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-31386
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
7570-GH
Species: Hu
NBP1-35703
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-22164
Species: Mu, Rt, Hu(-)
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP1-69750
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-60120
Species: Hu
Applications: WB, TCS
NB100-56350
Species: Hu, Mu, Rt
Applications: WB
NB300-174
Species: Hu, Mu, Rt, Bv
Applications: WB, Simple Western, IHC, IHC-P

Publications for Tryptophan hydroxylase 2 Antibody (NB100-74555)(17)

We have publications tested in 3 confirmed species: Mouse, Rat, Primate.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(7)
IHC
(7)
WB
(2)
All Applications
Filter By Species
Mouse
(7)
Rat
(2)
Primate
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-74555 Applications Species
Ma J, Wang F, Yang J et al. Xiaochaihutang attenuates depressive/anxiety-like behaviors of social isolation-reared mice by regulating monoaminergic system, neurogenesis and BDNF expression Journal of Ethnopharmacology 2017 Aug 17 [PMID: 28687505] (Mouse) Mouse
Natarajan R, Forrester L, Chiaia N, Yamamoto BK. Chronic Stress-induced Behavioral Changes Associated with Subregion Selective Serotonin Cell Death in the Dorsal Raphe. J. Neurosci. May 25 2017 [PMID: 28546314] (ICC/IF, Rat) ICC/IF Rat
Pomeranz LE, Ekstrand MI, Latcha KN et al. Gene expression profiling with Cre-conditional pseudorabies virus reveals a subset of midbrain neurons that participate in reward circuitry. J. Neurosci. Mar 10 2017 [PMID: 28283558] (IHC, Mouse) IHC Mouse
Goto S, Ogi H, Fushiki S, Itoh K. Prenatal and lactational bisphenol A exposure does not alter serotonergic neurons morphologically in the murine dorsal raphe nucleus. Brain Dev. 2017 Feb 21 [PMID: 28233694]
Coyle D, Murphy JM, Doyle B et al. Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung's-associated enterocolitis. World J Gastroenterol 2016 May 21 [PMID: 27217698] (WB) WB
Barrett KT, Dosumu-Johnson RT, Daubenspeck JA et al. Partial Raphe Dysfunction in Neurotransmission Is Sufficient to Increase Mortality after Anoxic Exposures in Mice at a Critical Period in Postnatal Development. J. Neurosci. 2016 Apr 06 [PMID: 27053202] (ICC/IF, Mouse) ICC/IF Mouse
Commons KG. Ascending serotonin neuron diversity under two umbrellas. Brain Struct Funct. 2016 Jan 06 [PMID: 26740230] (IHC, Mouse) IHC Mouse
Alter SP, Stout KA, Lohr KM et al. Reduced vesicular monoamine transport disrupts serotonin signaling but does not cause serotonergic degeneration. Exp Neurol 2016 Jan [PMID: 26428905] (IHC) IHC
Naslund J, Studer E, Petterson R et al. Differences in anxiety-like behaviour within a batch of Wistar rats are associated with differences in serotonergic transmission, enhanced by acute SSRI administration and abolished by serotonin depletion Int. J. Neuropsychopharmacol. 2015 Feb 25 [PMID: 25716782] (WB, Rat) WB Rat
Stansley BJ, Yamamoto BK. Chronic L-Dopa Decreases Serotonin Neurons in a Sub-region of the Dorsal Raphe Nucleus. J Pharmacol. Exp. Ther. 2014 Sep 11 [PMID: 25212217]
Show All 17 Publications.

Reviews for Tryptophan hydroxylase 2 Antibody (NB100-74555) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: ICC, IHC-P.

Reviews using NB100-74555:
Filter by Applications
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ICC Mouse 12/27/2011
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse
SpeciesMouse
CommentsExcellent specifity for serotonin neurons. Does not cross-react with tyrosine hydroxylase. Excellent signal/ noise.
Pub Med ID21550397

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Comments

CommentsExcellent specifity for serotonin neurons. Does not cross-react with tyrosine hydroxylase. Excellent signal/ noise.
Immunohistochemistry-Paraffin Tryptophan hydroxylase 2 NB100-74555
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 10/25/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain
SpeciesMouse
LotK1137602
CommentsTPH2 IHC staining in brain section of wild-type B6 adult mouse (P-45). Dilution of TPH2 Ab: 1:100. Secondary Ab - goat anti-rabbit Alexa-568 at 1:1000. Secondary Ab incubated for 1 hour at room temperature in the same buffer as the TPH2 Ab.

Blocking

Blocking DetailsBlocking Buffer: 10% Normal Goat Serum, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:100, Incubation Dilution Buffer: 1% goat serum, 0.1% tween-20 in PBS, Incubation Time/Temp: overnight at 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat-anto-rabbit Alexa 568, Secondary Ab Dilution: 1:1000

Details

Detection NotesMethod:fluorescent, Pos.Ctrl:NEUN, Neg.Ctrl:no primary, Deparaffinization:xylenes, Antigen Retrieval:Tris-EDTA pH9, 10min @ 100C
Fixation DetailsSample Preparation: 4% PFA fixed & Paraffin embedded, Deparaffinization: xylenes

Comments

CommentsTPH2 IHC staining in brain section of wild-type B6 adult mouse (P-45). Dilution of TPH2 Ab: 1:100. Secondary Ab - goat anti-rabbit Alexa-568 at 1:1000. Secondary Ab incubated for 1 hour at room temperature in the same buffer as the TPH2 Ab.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tryptophan hydroxylase 2 Antibody (NB100-74555) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Tryptophan hydroxylase 2 Products

Bioinformatics Tool for Tryptophan hydroxylase 2 Antibody (NB100-74555)

Discover related pathways, diseases and genes to Tryptophan hydroxylase 2 Antibody (NB100-74555). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Tryptophan hydroxylase 2 Antibody (NB100-74555)

Discover more about diseases related to Tryptophan hydroxylase 2 Antibody (NB100-74555).
 

Pathways for Tryptophan hydroxylase 2 Antibody (NB100-74555)

View related products by pathway.

PTMs for Tryptophan hydroxylase 2 Antibody (NB100-74555)

Learn more about PTMs related to Tryptophan hydroxylase 2 Antibody (NB100-74555).
 

Research Areas for Tryptophan hydroxylase 2 Antibody (NB100-74555)

Find related products by research area.

Blogs on Tryptophan hydroxylase 2

There are no specific blogs for Tryptophan hydroxylase 2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
12/27/2011
Application: ICC
Species: Mouse

Anonymous
10/25/2010
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol TPH2
Entrez
Uniprot