Tryptophan hydroxylase 2 Antibody

Images

 
Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody [NB100-74555] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using Tryptophan hydroxylase 2 antibody at 1:25 dilution. ...read more
Western Blot: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Total protein from mouse brain, stomach and human brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - The 5-HT and Tryptophan hydroxylase 2 in the raphe nuclei of the brain. The 5-HT- and Tryptophan ...read more
Immunocytochemistry/ Immunofluorescence: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis in mouse brain cells. Image courtesy of product review by Assaf Vestin.
Immunohistochemistry Free-Floating: Tryptophan hydroxylase 2 Antibody [NB100-74555] - PFA-perfused mouse midbrain serotoninergic neurons. AlexaFluor488 used as secondary antibody. Neurons pseudocolored in yellow hot ...read more
Immunoprecipitation: Tryptophan hydroxylase 2 Antibody [NB100-74555] - Analysis of raphe nuclei isolated from Sprague-Dawley rat brains using TPH2 Polyclonal Antibody. PA1-778 was coupled to magnetic beads and added to ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Tryptophan hydroxylase 2 Antibody Summary

Immunogen
Synthetic peptide make to an internal portion of the mouse Tryptophan hydroxylase 2 protein (between amino acids 10-75) [UniProt Q8CGV2]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:1000
  • Immunohistochemistry Free-Floating 1:10 - 1:500
Application Notes
IF usage was reported in scientific literature (see Soiza-Reillly M et al, 2011). Use in IHC-FF reported in scientific literature (PMID: 26428905). Use in FLOW reported in scientific literature (PMID: 30581079). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 30350781).
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB100-74555 in the following applications:

Publications
Read Publications using
NB100-74555 in the following applications:

Reactivity Notes

Human reactivity is weak. Mouse reactivity reported in scientific literature (PMID: 16581041). Rat reactivity reported in scientific literature (PMID: 24066056).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Tryptophan hydroxylase 2 Antibody

  • ADHD7
  • EC 1.14.16
  • EC 1.14.16.4
  • FLJ37295
  • MGC138872
  • Neuronal tryptophan hydroxylase
  • NTPH
  • NTPHMGC138871
  • TPH2
  • tryptophan 5-hydroxylase 2
  • Tryptophan 5-monooxygenase 2
  • Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tryptophan hydroxylase 2 Antibody (NB100-74555)(34)

We have publications tested in 3 confirmed species: Mouse, Rat, Primate.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
Flow
(1)
ICC/IF
(10)
IHC
(13)
IHC-Fr
(2)
WB
(2)
All Applications
Filter By Species
Mouse
(19)
Rat
(2)
Primate
(2)
All Species
Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using NB100-74555 Applications Species
Seigneur E, Wang J, Dai J et al. Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors Molecular psychiatry Jun 22 2021 [PMID: 34158618]
Konig N, Bimpisidis Z, Dumas S, Wallen-Mackenzie A Selective Knockout of the Vesicular Monoamine Transporter 2 (Vmat2) Gene in Calbindin2/Calretinin-Positive Neurons Results in Profound Changes in Behavior and Response to Drugs of Abuse Sci Transl Med Nov 25 2020 [PMID: 33240055]
Okaty BW, Sturrock N, Escobedo Lozoya Y et al. A single-cell transcriptomic and anatomic atlas of mouse dorsal raphe Pet1 neurons Elife Jun 22 2020 [PMID: 32568072] (ICC/IF, Mouse) ICC/IF Mouse
Li Y, Li CY, Xi W et al. Rostral and Caudal Ventral Tegmental Area GABAergic Inputs to Different Dorsal Raphe Neurons Participate in Opioid Dependence Neuron Feb 20 2019 [PMID: 30638902] (IHC, Mouse) IHC Mouse
Thier MC, Hommerding O, Panten J et al. Identification of Embryonic Neural Plate Border Stem Cells and Their Generation by Direct Reprogramming from Adult Human Blood Cells Cell Stem Cell Dec 12 2018 [PMID: 30581079] (Flow, Mouse) Flow Mouse
Dosumu-Johnson RT, Cocoran AE, Chang Y et al. Acute perturbation of Pet1-neuron activity in neonatal mice impairs cardiorespiratory homeostatic recovery. Elife. Oct 23 2018 [PMID: 30350781] (IHC-Fr, Mouse) IHC-Fr Mouse
Soiza-Reilly M, Meye FJ, Olusakin J et al. SSRIs target prefrontal to raphe circuits during development modulating synaptic connectivity and emotional behavior. Mol. Psychiatry. Oct 2 2018 [PMID: 30279456] (IHC, Mouse) IHC Mouse
Ehlinger DG, Commons KG. Cav1.2L-type calcium channels regulate stress coping behavior via serotonin neurons. Neuropharmacology Aug 31 2018 [PMID: 30176250] (ICC/IF, Mouse) ICC/IF Mouse
Huerta-Ocampo I, Dautan D, Gut N et al. Whole-brain mapping of monosynaptic inputs to midbrain cholinergic neurons Scientific Reports Dec 1 2021
Vieira JS, de Souza GR, Kalil-Cutti B et al. Post-traumatic stress disorder increases pain sensitivity by reducing descending noradrenergic and serotoninergic modulation Behavioural brain research May 14 2021 [PMID: 34000338]
Show All 34 Publications.

Reviews for Tryptophan hydroxylase 2 Antibody (NB100-74555) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using NB100-74555:
Filter by Applications
IHC-Fr
(1)
ICC
(1)
IHC-P
(1)
All Applications
Filter by Species
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Tryptophan hydroxylase 2 NB100-74555
Enlarge
5
reviewed by:
Luiz Alexandre Magno
IHC-Fr Mouse 07/19/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmidbrain
SpeciesMouse
Lot1068-102

Comments

CommentsThis is a fantastic antibody to detect TPH2 in free floating slices from PFA-perfused mice. I use it with Alexa Fluor 488 2nd antibody.
  5
reviewed by:
Verified Customer
ICC Mouse 12/27/2011
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse
SpeciesMouse
CommentsExcellent specifity for serotonin neurons. Does not cross-react with tyrosine hydroxylase. Excellent signal/ noise.
Pub Med ID21550397

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Comments

CommentsExcellent specifity for serotonin neurons. Does not cross-react with tyrosine hydroxylase. Excellent signal/ noise.
Immunohistochemistry-Paraffin Tryptophan hydroxylase 2 NB100-74555
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 10/25/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain
SpeciesMouse
LotK1137602
CommentsTPH2 IHC staining in brain section of wild-type B6 adult mouse (P-45). Dilution of TPH2 Ab: 1:100. Secondary Ab - goat anti-rabbit Alexa-568 at 1:1000. Secondary Ab incubated for 1 hour at room temperature in the same buffer as the TPH2 Ab.

Blocking

Blocking DetailsBlocking Buffer: 10% Normal Goat Serum, Blocking Time: 30 minutes, Blocking Temp: room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:100, Incubation Dilution Buffer: 1% goat serum, 0.1% tween-20 in PBS, Incubation Time/Temp: overnight at 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: goat-anto-rabbit Alexa 568, Secondary Ab Dilution: 1:1000

Details

Detection NotesMethod:fluorescent, Pos.Ctrl:NEUN, Neg.Ctrl:no primary, Deparaffinization:xylenes, Antigen Retrieval:Tris-EDTA pH9, 10min @ 100C
Fixation DetailsSample Preparation: 4% PFA fixed & Paraffin embedded, Deparaffinization: xylenes

Comments

CommentsTPH2 IHC staining in brain section of wild-type B6 adult mouse (P-45). Dilution of TPH2 Ab: 1:100. Secondary Ab - goat anti-rabbit Alexa-568 at 1:1000. Secondary Ab incubated for 1 hour at room temperature in the same buffer as the TPH2 Ab.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Tryptophan hydroxylase 2 Antibody (NB100-74555) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-74555AF350
Alexa Fluor 405 NB100-74555AF405
Alexa Fluor 488 NB100-74555AF488
Alexa Fluor 532 NB100-74555AF532
Alexa Fluor 594 NB100-74555AF594
Alexa Fluor 647 NB100-74555AF647
Alexa Fluor 700 NB100-74555AF700
Alexa Fluor 750 NB100-74555AF750
Biotin NB100-74555B
DyLight 350 NB100-74555UV
DyLight 405 NB100-74555V
DyLight 488 NB100-74555G
DyLight 550 NB100-74555R
DyLight 594 NB100-74555DL594
DyLight 650 NB100-74555C
DyLight 680 NB100-74555FR
DyLight 755 NB100-74555IR
FITC NB100-74555F
HRP NB100-74555H
Janelia Fluor 549 NB100-74555JF549
Janelia Fluor 646 NB100-74555JF646

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Luiz Alexandre Magno
07/19/2018
Application: IHC-Fr
Species: Mouse

 
Verified Customer
12/27/2011
Application: ICC
Species: Mouse

Verified Customer
10/25/2010
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol TPH2
Entrez
Uniprot