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Dopa Decarboxylase/DDC Antibody

 (1 Publication)
 
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Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.
Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.
Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.
Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Dopa Decarboxylase/DDC Antibody Summary

Immunogen
SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies.
Specificity
Specific for the ~55k DDC protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody can be used in Western blot where a single band at 55 kDa is seen. Immunohistochemistry-Paraffin was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB300-174 in the following applications:

  • IHC-P
    1 publication
  • WB
    1 publication

Reactivity Notes

Porcine reactivity reported in (PMID: 19857468).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Dopa Decarboxylase/DDC Antibody

  • AADC
  • AADCaromatic-L-amino-acid decarboxylase
  • DDC
  • dopa decarboxylase (aromatic L-amino acid decarboxylase)
  • Dopa Decarboxylase
  • EC 4.1.1
  • EC 4.1.1.28

Background

DOPA decarboxylase (DDC) is responsible for the synthesis of the neurotransmitters dopamine and serotonin. These two neurotransmitters play key roles in many clinical disorders, including Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Decarboxylase/DDC Ant
NB300-174
Dopa Decarboxylase/DDC Antibody
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, Simple Western, IHC, IHC-P

Publications for Dopa Decarboxylase/DDC Antibody (NB300-174)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 2 applications: IHC-P, WB.


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Bioinformatics

Gene Symbol DDC
Entrez
Uniprot