Dopa Decarboxylase/DDC Antibody

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla. The antibody is specific for the ~55 kDa DOPA decarboxylase protein.

Western Blot: Dopa Decarboxylase/DDC Antibody [NB300-174] - Rat adrenal medulla showing specific immunolabeling of the ~55k DDC protein.

Simple Western: Dopa Decarboxylase/DDC Antibody [NB300-174] - Simple Western lane view shows a specific band for DOPA Decarboxylase in 0.2 mg/ml of Mouse Cerebellum lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, Simple Western, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Dopa Decarboxylase/DDC Antibody Summary

• Immunogen: SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies.
• Specificity: Specific for the ~55k DDC protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This antibody can be used in Western blot where a single band at 55 kDa is seen. Immunohistochemistry-Paraffin was reported in scientific literature.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in (PMID: 19857468).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for Dopa Decarboxylase/DDC Antibody

• AADC
• AADCaromatic-L-amino-acid decarboxylase
• DDC
• dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Dopa Decarboxylase
• EC 4.1.1
• EC 4.1.1.28

Background

DOPA decarboxylase (DDC) is responsible for the synthesis of the neurotransmitters dopamine and serotonin. These two neurotransmitters play key roles in many clinical disorders, including Parkinson's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dopa Decarboxylase/DDC Antibody (NB300-174)(1)

Publication using NB300-174

• Blechingberg J, Holm IE, Johansen MG et al. Aromatic l-amino acid decarboxylase expression profiling and isoform detection in the developing porcine brain. Brain Res 2010 Jan 13 [PMID: 19857468] (WB, IHC-P, Porcine)

Bioinformatics

• Gene Symbol: DDC
• Entrez:
  • 24311(Rat)
  • 280762(Bovine)
• Uniprot:
  • P27718(Bovine)