Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the basal forebrain.

Immunohistochemistry: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of mouse brain shows moderate positivity in serotonergic fibers in the cerebral cortex.

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.

Immunohistochemistry-Paraffin: Tryptophan hydroxylase 2 Antibody (CL2990) [NBP2-46646] - Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL2990
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free Summary

• Description: Novus Biologicals Mouse Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free (NBP2-46646) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q8IWU9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  Tryptophan hydroxylase 2 Recombinant Protein Antigen (NBP2-46646PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Tryptophan hydroxylase 2 Antibody (CL2990) - BSA Free

• ADHD7
• EC 1.14.16
• EC 1.14.16.4
• FLJ37295
• MGC138872
• Neuronal tryptophan hydroxylase
• NTPH
• NTPHMGC138871
• TPH2
• tryptophan 5-hydroxylase 2
• Tryptophan 5-monooxygenase 2
• Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPH2
• Entrez:
  • 121278(Human)
• Uniprot:
  • Q8IWU9(Human)