Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

Staining of human kidney shows no positivity in cells in tubules or glomeruli as expected.

Staining of human dorsal raphe nucleus shows strong cytoplasmic positivity in serotonin neurons.

Staining of human cerebral cortex shows moderate positivity in serotonergic neural fibers.

Staining of mouse brain shows moderate positivity in serotonergic fibers in the cerebral cortex.

Staining of rat midbrain shows strong positivity in serotonin neurons in the dorsal raphe nucleus.

Staining of mouse brain shows moderate positivity in serotonergic fibers in the basal forebrain.

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: CL2990
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free (NBP2-46646) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q8IWU9, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Tryptophan hydroxylase 2 Antibody (CL2990) - Azide and BSA Free

• ADHD7
• EC 1.14.16
• EC 1.14.16.4
• FLJ37295
• MGC138872
• Neuronal tryptophan hydroxylase
• NTPH
• NTPHMGC138871
• TPH2
• tryptophan 5-hydroxylase 2
• Tryptophan 5-monooxygenase 2
• Tryptophan Hydroxylase 2

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPH2