TRPM2 Antibody

Western Blot: TRPM2 Antibody [NB500-241] - Membrane Prep (50 ug) from Jurkat cells. Antibody: Affinity purified rabbit anti-TRPM2 antibody used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: TRPM2 Antibody [NB500-241] - Detection of human TRPM2 by western blot of immunoprecipitates. Samples: Membrane Prep (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TRPM2 antibody NB500-241 used for IP at 6 ug per reaction. TRPM2 was also immunoprecipitated by rabbit anti-TRPM2 antibody NB500-242. For blotting immunoprecipitated TRPM2, NB500-241 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Product Discontinued

TRPM2 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1200 and 1250 of human Transient Receptor Potential Cation Channel, Subfamily M, Member 2 using the numbering given in entry NP_003298.1 (GeneID 7226).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRPM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg lysate
  • SDS-Page
  • Western Blot 1:500
• Application Notes: Use in Page reported in scientific literature (PMID:35328585) Western Blot reported in scientific literature (PMID: 21753080)..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPM2 Antibody

• EC 3.6.1.13
• EREG1MGC133383
• Estrogen-responsive element-associated gene 1 protein
• KNP3LTrpC-2
• Long transient receptor potential channel 2
• LTrpC2
• LTRPC2TRPC7transient receptor potential cation channel subfamily M member 2
• NUDT9H
• NUDT9L1
• transient receptor potential cation channel, subfamily M, member 2
• Transient receptor potential channel 7
• TrpC7

Background

TRPM2 is a Ca(2+)-permeable channel that is activated by oxidative stress and confers susceptibility to cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPM2 Antibody (NB500-241)(5)

Publications using NB500-241

• Sander S, Gattkowski E, Pick J et al. The crystal structure of TRPM2 MHR1/2 domain reveals a conserved Zn2+-binding domain essential for ligand binding and activity Protein Sci 2022-05-31 [PMID: 35634784]
• Riekehr WM, Sander S, Pick J et al. cADPR Does Not Activate TRPM2 International journal of molecular sciences 2022-03-15 [PMID: 35328585] (PAGE)
• Gattkowski E, Johnsen A, Bauche A et al. Novel CaM-binding motif in its NudT9H domain contributes to temperature sensitivity of TRPM2 Biochim Biophys Acta Mol Cell Res 2018-12-22 [PMID: 30584900] (WB, Human)
• Moreau C, Kirchberger T, Swarbrick JM et al. Structure-Activity Relationship of Adenosine 5'-diphosphoribose at the Transient Receptor Potential Melastatin 2 (TRPM2) Channel: Rational Design of Antagonists. J Med Chem 2013-12-27 [PMID: 24304219] (WB, Human)
• Partida-Sanchez, S et al. Chemotaxis of Mouse Bone Marrow Neutrophils Dendritic Cells is Controlled by ADP-Ribose, the Major Product Generated by the CD38 Enzyme Reaction. J Immunol; 179: 7827-7839. 2007-12-01 [PMID: 18025229]

Bioinformatics

• Gene Symbol: TRPM2
• Entrez:
  • 7226(Human)
• Uniprot:
  • O94759(Human)