TRPC6 Antibody

Western Blot: TRPC6 Antibody [NB600-791] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Flow Cytometry: TRPC6 Antibody [NB600-791] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Po, Bv, Ca
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRPC6 Antibody Summary

• Immunogen: Peptide with sequence C-EKLSMEPNQEETN corresponding to C-Terminus according to NP_004612.2.
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRPC6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: Western blot: Approx 100 kDa band observed in lysates of cell line A549 (calculated MW of 106 kDa according to NP_004612.2). Preliminary experiments also showed 100 kDa in Human Lung lysates and in lysates of cell line HEK293 .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRPC6 Antibody

• FLJ11098
• FSGS2
• short transient receptor potential channel 6
• transient receptor potential cation channel, subfamily C, member 6
• Transient receptor protein 6
• TRP6
• TRP-6
• TRP6FLJ14863
• TRPC6

Background

The protein encoded by the TRPC6 gene creates receptor-activated cation permeant calcium channels in the cell membrane. Isoform 1 is 931 amino acids in length and is approximately 106 kDa, while isoform 2 is 815 amino acids in lenth at around 93 kDA. Isoform 3 is 876 amino acids long and is just over 100 kDA. Focal segmental glomerulosclerosis 2 (FSGS2) is caused by deficits in the TRPC6 gene. This gene has been linked to diseases such as cystic fibrosis, gigantism, hypertension, kidney disease, glomerulosclerosis, pyloric stenosis, diencephalic neoplasm, premature ovarian failure, and hepatopulmonary syndrome. The TRPC6 gene interacts with MX1, ORAI1, NPHS1, NPHS2, and FYN genes in pathways that monitor sodium as well as calcium channels, EPO signaling pathway, G alpha (q) signaling events, signal transduction, and platelet homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPC6 Antibody (NB600-791)(1)

Publication using NB600-791

• Winn MP, Conlon PJ, Lynn KL et al. A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis. Science 2005-06-17 [PMID: 15879175]

Bioinformatics

• Gene Symbol: TRPC6
• Entrez:
  • 7225(Human)
  • 22068(Mouse)
• Uniprot:
  • NP_004612.2(Human)