TRPC6 Antibody (3F2.H10.F2)

Images

 
Western Blot: TRPC6 Antibody (3F2.H10.F2) [NBP1-42738] - Lane 1: Mouse Kidney WCL Load: 10 ug per lane Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4C Secondary antibody: DyLight 649 donkey anti-mouse at ...read more
Immunohistochemistry: TRPC6 Antibody (3F2.H10.F2) [NBP1-42738] - Analysis of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL. The image shows strong staining with minimal ...read more

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
3F2.H10.F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TRPC6 Antibody (3F2.H10.F2) Summary

Immunogen
This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
Specificity
A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% identity with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TRPC6
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 106 kDa in size corresponding - TRPC6 protein by western blotting in the appropriate cell lysate or extract. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal - no background staining and appeared very specific at 2.5 ug/mL.

Reactivity Notes

human, chimpanzee

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for TRPC6 Antibody (3F2.H10.F2)

  • FLJ11098
  • FSGS2
  • short transient receptor potential channel 6
  • transient receptor potential cation channel, subfamily C, member 6
  • Transient receptor protein 6
  • TRP-6
  • TRP6FLJ14863
  • TrpC6

Background

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TRPC6
Entrez