TRPC6 Antibody (3F2.H10.F2)

Western Blot: TRPC6 Antibody (3F2.H10.F2) [NBP1-42738] - Lane 1: Mouse Kidney WCL Load: 10 ug per lane Primary antibody: TRPC6 Antibody at 1:1000 for overnight at 4C Secondary antibody: DyLight 649 donkey anti-mouse at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT

Immunohistochemistry: TRPC6 Antibody (3F2.H10.F2) [NBP1-42738] - Analysis of TRPC6 in human adrenal (cortex) tissue (40X). The antibody was used a dilution to 2.5 ug/mL. The image shows strong staining with minimal background staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Product Discontinued

TRPC6 Antibody (3F2.H10.F2) Summary

• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
• Specificity: A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% identity with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRPC6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:50000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: Expect a band approximately 106 kDa in size corresponding - TRPC6 protein by western blotting in the appropriate cell lysate or extract. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. Strong staining was observed in adrenal, Purkinje neurons, cortical neurons, heart, ganglion cells, renal tubules, Sertoli cells, hepatocytes, skeletal muscle, exocrine pancreas, and germinal centers of lymphoid follicles. Moderate staining was observed in colon epithelium and plasma cells, B-lymphocytes, and parafollicular cells of the thyroid. Faint staining was seen in respiratory epithelium. Prostate and placenta were negative for staining. The antibody produced minimal - no background staining and appeared very specific at 2.5 ug/mL.

Reactivity Notes

Chimpanzee 100%

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TRPC6 Antibody (3F2.H10.F2)

• FLJ11098
• FSGS2
• short transient receptor potential channel 6
• transient receptor potential cation channel, subfamily C, member 6
• Transient receptor protein 6
• TRP6
• TRP-6
• TRP6FLJ14863
• TRPC6

Background

TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRPC6
• Entrez:
  • 7225(Human)