IP3R3 Antibody - BSA Free

Immunoprecipitation: IP3R3 Antibody [NBP3-03498] - Analysis of 300 μg extracts of Hela cells using 3 μg ITPR3 antibody. Western blot was performed from the immunoprecipitate using ITPR3 antibody at a dilution of 1:1000.

Western Blot: IP3R3 Antibody [NBP3-03498] - Analysis of lysates from HeLa cells, using ITPR3 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: IP3R3 Antibody - Azide and BSA Free [IP3R3] - Immunofluorescence analysis of NIH-3T3 cells using IP3R3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IP3R3 Antibody - BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1870 of human IP3R3 (NP_002215.2). EGSEAYQRCESGGFLSKLIQHTKDLMESEEKLCIKVLRTLQQMLLKKTKYGDRGNQLRKMLLQNYLQNRKSTSRGDLPDPIGTGLDPDWSAIAATQCRLDKEGATKLVCDLITSTKNEKIFQESIGLAIHLLDGGNTEIQKSFHNLMMSDKKSERFFKVLHDRMKRAQQETKSTVAVNMNDLGSQPHEDREPVDPTTKGRVASFSIPGSSSRYSLGPSLRRGHEVSERVQSSEMGTSVLIM
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ITPR3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 304 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for IP3R3 Antibody - BSA Free

• FLJ36205
• inositol 14,5-triphosphate receptor, type 3
• inositol 14,5-trisphosphate receptor type 3
• InsP3R3
• IP3 receptor isoform 3
• IP3 receptor
• IP3R 3
• IP3R
• IP3R3
• ITPR3
• Type 3 inositol 14,5-trisphosphate receptor
• Type 3 InsP3 receptor

Background

IP3R3 (inositol 1,4,5-triphosphate receptor type 3) is an intracellular calcium release channel that mediates calcium release from the endoplasmic reticulum. There are 3 mammalian IP3R receptors: IP3R1, IP3R2, and IP3R3. IP3R3 has been shown to play a role in olfactory mucus secretion and in the taste perception of sweet, savory (umami), and bitter substances.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: ITPR3